UniProt Accession

 MRP_HUMAN; P49006; D3DPQ0; Q5TEE6; Q6NXS5 

Genbank Protein ID

 X70326; AL109945; AY293577; AL713653; CH471059; CH471059; BC007904; BC066915 

Genbank Nucleotide ID

 CAA49793.1; CAI22887.1; AAP37955.1; CAD28462.1; EAX07538.1; EAX07539.1; AAH07904.1; AAH66915.1 

Protein Name

 MARCKS-related protein 

Protein Synonyms/Alias

 MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; Mac-MARCKS; MacMARCKS 

Gene Name

 MARCKSL1 

Gene Synonyms/Alias

 MLP; MRP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	AAGASPAKANGQENG	ubiquitination	[1, 2, 3]
35	GQENGHVKSNGDLSP	ubiquitination	[2]
43	SNGDLSPKGEGESPP	ubiquitination	[1, 4]
77	PSQGAEAKGEVPPKE	ubiquitination	[1]
95	KKKKFSFKKPFKLSG	ubiquitination	[3]
99	FSFKKPFKLSGLSFK	ubiquitination	[5, 6, 7]
106	KLSGLSFKRNRKEGG	acetylation	[8]
106	KLSGLSFKRNRKEGG	ubiquitination	[2, 5, 6, 7]
110	LSFKRNRKEGGGDSS	ubiquitination	[1, 3]
144	EGTAQEGKAAATPES	phosphoglycerylation	[9]
144	EGTAQEGKAAATPES	ubiquitination	[1]
157	ESQEPQAKGAEASAA	ubiquitination	[1, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [9] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

 Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity). 

Sequence Annotation

 REGION 87 110 Effector domain involved in lipid-binding
 REGION 87 100 Calmodulin-binding (PSD).
 MOD_RES 22 22 Phosphoserine.
 MOD_RES 36 36 Phosphoserine.
 MOD_RES 41 41 Phosphoserine.
 MOD_RES 48 48 Phosphoserine (By similarity).
 MOD_RES 71 71 Phosphoserine.
 MOD_RES 85 85 Phosphothreonine (By similarity).
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 104 104 Phosphoserine.
 MOD_RES 120 120 Phosphoserine (By similarity).
 MOD_RES 135 135 Phosphoserine (By similarity).
 MOD_RES 148 148 Phosphothreonine.
 MOD_RES 151 151 Phosphoserine.
 MOD_RES 162 162 Phosphoserine (By similarity).
 MOD_RES 165 165 Phosphoserine (By similarity).
 MOD_RES 178 178 Phosphothreonine.
 MOD_RES 180 180 Phosphoserine (By similarity).
 MOD_RES 187 187 Phosphothreonine (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 Actin-binding; Calmodulin-binding; Cell membrane; Complete proteome; Cytoplasm; Lipoprotein; Membrane; Myristate; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 195 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:LIFEdb.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara. 

Interpro

 IPR002101; MARCKS. 

Pfam

 PF02063; MARCKS 

SMART

  

PROSITE

 PS00826; MARCKS_1
 PS00827; MARCKS_2 

PRINTS

 PR00963; MARCKS.