UniProt Accession

 TIM10_HUMAN; P62072; A8K136; Q9WV99; Q9WVA0; Q9Y5J8 

Genbank Protein ID

 AF150089; AF152354; AK289751; CH471076; BC032133 

Genbank Nucleotide ID

 AAD39995.1; AAF15104.1; BAF82440.1; EAW73753.1; AAH32133.1 

Protein Name

 Mitochondrial import inner membrane translocase subunit Tim10 

Protein Synonyms/Alias

  

Gene Name

 TIMM10 

Gene Synonyms/Alias

 TIM10 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
45	YKEAELSKGESVCLD	ubiquitination	[1, 2]
57	CLDRCVSKYLDIHER	ubiquitination	[1, 2, 3]
81	MQDEELMKRVQQSSG	ubiquitination	[1, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. 

Sequence Annotation

 MOTIF 29 54 Twin CX3C motif.
 DISULFID 29 54
 DISULFID 33 50  

Keyword

 3D-structure; Chaperone; Complete proteome; Disulfide bond; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Protein transport; Reference proteome; Translocation; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 90 AA 

Protein Sequence

Gene Ontology

 GO:0005744; C:mitochondrial inner membrane presequence translocase complex; TAS:UniProtKB.
 GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IDA:BHF-UCL.
 GO:0005215; F:transporter activity; IDA:BHF-UCL.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0072321; P:chaperone-mediated protein transport; IDA:BHF-UCL.
 GO:0045039; P:protein import into mitochondrial inner membrane; IDA:BHF-UCL.
 GO:0007605; P:sensory perception of sound; TAS:UniProtKB. 

Interpro

 IPR004217; Tim10/DDP_fam_Znf.
 IPR027247; Tim10/Tim12. 

Pfam

 PF02953; zf-Tim10_DDP 

SMART

  

PROSITE

  

PRINTS