UniProt Accession

 OTU1_HUMAN; Q5VVQ6; B2RNX3; Q5VVQ5; Q6ZRS6; Q86T63; Q9P1L8 

Genbank Protein ID

 AK128014; AL445493; AL445493; CH471100; CH471100; BC137166; BC137167; AL833081; AF116608 

Genbank Nucleotide ID

 BAC87233.1; CAH70775.1; CAH70776.1; EAW93509.1; EAW93510.1; AAI37167.1; AAI37168.1; CAD89975.1; AAF71033.1 

Protein Name

 Ubiquitin thioesterase OTU1 

Protein Synonyms/Alias

 DUBA-8; HIV-1-induced protease 7; HIN-7; HsHIN7; OTU domain-containing protein 2 

Gene Name

 YOD1 

Gene Synonyms/Alias

 DUBA8; HIN7; OTUD2; PRO0907 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
54	WRLRCKAKDGTHVLQ	ubiquitination	[1]
135	RSSPAFTKRGASSYV	ubiquitination	[2]
257	GEDAGYTKRVLLIYD	ubiquitination	[1, 2, 3, 4]
325	LRCMVCQKGLTGQAE	ubiquitination	[2, 5]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Able to cleave both polyubiquitin and di- ubiquitin. 

Sequence Annotation

 DOMAIN 149 274 OTU.
 ZN_FING 318 342 C2H2-type.
 REGION 50 128 UBX-like.
 ACT_SITE 157 157 By similarity.
 ACT_SITE 160 160 Nucleophile (Probable).
 ACT_SITE 342 342 By similarity.  

Keyword

 Alternative splicing; Complete proteome; Hydrolase; Metal-binding; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway; Unfolded protein response; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 348 AA 

Protein Sequence

Gene Ontology

 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; ISS:UniProtKB.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:UniProtKB.
 GO:0030433; P:ER-associated protein catabolic process; IMP:UniProtKB.
 GO:0071108; P:protein K48-linked deubiquitination; IDA:UniProtKB.
 GO:0070536; P:protein K63-linked deubiquitination; IDA:UniProtKB. 

Interpro

 IPR003323; OTU.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 

Pfam

 PF02338; OTU 

SMART

 SM00355; ZnF_C2H2 

PROSITE

 PS50802; OTU
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS