UniProt Accession

 SKA1_HUMAN; Q96BD8; B2R9Y6; B4E0P4 

Genbank Protein ID

 AK303464; AK313968; CH471096; BC015706 

Genbank Nucleotide ID

 BAG64506.1; BAG36683.1; EAW62971.1; AAH15706.1 

Protein Name

 Spindle and kinetochore-associated protein 1 

Protein Synonyms/Alias

  

Gene Name

 SKA1 

Gene Synonyms/Alias

 C18orf24 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
153	NGVPSYMKSRLTYNQ	ubiquitination	[1]
166	NQINDVIKEINKAVI	ubiquitination	[1]
175	INKAVISKYKILHQP	ubiquitination	[1]
203	RFIDEETKDTKGRYF	ubiquitination	[1]
206	DEETKDTKGRYFIVE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HPA.
 GO:0000940; C:condensed chromosome outer kinetochore; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005876; C:spindle microtubule; IDA:UniProtKB.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0051301; P:cell division; IMP:UniProtKB.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0000090; P:mitotic anaphase; IMP:UniProtKB.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0031110; P:regulation of microtubule polymerization or depolymerization; IDA:UniProtKB. 

Interpro

 IPR009829; DUF1395. 

Pfam

 PF07160; DUF1395 

SMART

  

PROSITE

  

PRINTS