UniProt Accession

 UB2L3_MOUSE; P68037; P51966; P70653; Q9HAV1 

Genbank Protein ID

 S81004; X97042; AJ130961; AK007265 

Genbank Nucleotide ID

 AAB36018.1; CAA65755.1; CAA10265.1; BAB24925.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 L3 

Protein Synonyms/Alias

 UbcM4; Ubiquitin carrier protein L3; Ubiquitin-protein ligase L3 

Gene Name

 Ube2l3 

Gene Synonyms/Alias

 Ubce7 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
64	FPAEYPFKPPKITFK	ubiquitination	[1]
67	EYPFKPPKITFKTKI	ubiquitination	[1]
73	PKITFKTKIYHPNID	ubiquitination	[1]
82	YHPNIDEKGQVCLPV	ubiquitination	[1]
96	VISAENWKPATKTDQ	ubiquitination	[1]
131	DLAEEYSKDRKKFCK	acetylation	[2]
131	DLAEEYSKDRKKFCK	ubiquitination	[1]
138	KDRKKFCKNAEEFTK	acetylation	[2]
138	KDRKKFCKNAEEFTK	ubiquitination	[1]
145	KNAEEFTKKYGEKRP	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis (By similarity). 

Sequence Annotation

 ACT_SITE 86 86 Glycyl thioester intermediate (By
 MOD_RES 9 9 N6-acetyllysine (By similarity).
 MOD_RES 131 131 N6-acetyllysine (By similarity).
 MOD_RES 138 138 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Nucleus; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 154 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; ISS:UniProtKB.
 GO:0071385; P:cellular response to glucocorticoid stimulus; ISS:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS