UniProt Accession

 HINT_ECOLI; P0ACE7; P36950; P75945 

Genbank Protein ID

 U00096; AP009048; X17615 

Genbank Nucleotide ID

 AAC74187.1; BAA35910.1 

Protein Name

 HIT-like protein HinT 

Protein Synonyms/Alias

 Purine nucleoside phosphoramidase 

Gene Name

 hinT 

Gene Synonyms/Alias

 ycfF; b1103; JW1089 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
9	AEETIFSKIIRREIP	acetylation	[1]
71	RMITVAAKIAEQEGI	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Hydrolyzes purine nucleotide phosphoramidates, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate), guanosine 5'monophosphomorpholidate (GMP-morpholidate) and tryptamine 5'guanosine monophosphate (TpGd). Hydrolyzes lysyl-AMP (AMP-N- epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase and lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)). Required for growth at high salt concentrations. Required for normal DadA activity and the ability to grow with D,L-alanine as carbon source. 

Sequence Annotation

 DOMAIN 6 115 HIT.
 NP_BIND 30 32 Purine nucleotide phosphoramidate.
 NP_BIND 96 97 Purine nucleotide phosphoramidate.
 NP_BIND 101 103 Purine nucleotide phosphoramidate.
 MOTIF 99 103 Histidine triad motif.
 ACT_SITE 101 101 Tele-AMP-histidine intermediate.
 BINDING 88 88 Purine nucleotide phosphoramidate.  

Keyword

 3D-structure; Complete proteome; Hydrolase; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 119 AA 

Protein Sequence

Gene Ontology

 GO:0043530; F:adenosine 5'-monophosphoramidase activity; IDA:EcoCyc.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0055130; P:D-alanine catabolic process; IMP:EcoCyc. 

Interpro

 IPR019808; Histidine_triad_CS.
 IPR001310; Histidine_triad_HIT.
 IPR011146; HIT-like. 

Pfam

 PF01230; HIT 

SMART

  

PROSITE

 PS00892; HIT_1
 PS51084; HIT_2 

PRINTS

 PR00332; HISTRIAD.