Tag | Content |
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CPLM ID | CPLM-001216 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Polycomb protein EED |
Protein Synonyms/Alias | hEED; WD protein associating with integrin cytoplasmic tails 1; WAIT-1 |
Gene Name | EED |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
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197 | GNAINELKFHPRDPN | ubiquitination | [1] | 211 | NLLLSVSKDHALRLW | ubiquitination | [1] | 275 | KRMMNAIKESYDYNP | ubiquitination | [2] | 284 | SYDYNPNKTNRPFIS | ubiquitination | [1, 2] | 433 | CTTLTHHKCGAAIRQ | ubiquitination | [1] |
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Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties. Chen Z, Zhou Y, Song J, Zhang Z. Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [ PMID: 23603789] |
Functional Description | Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. |
Sequence Annotation | REPEAT 91 134 WD 1. REPEAT 142 185 WD 2. REPEAT 188 228 WD 3. REPEAT 234 275 WD 4. REPEAT 304 341 WD 5. REPEAT 359 399 WD 6. REPEAT 408 441 WD 7. REGION 81 441 Interaction with EZH2 (By similarity). REGION 149 303 Required for interaction with the matrix REGION 301 441 Required for interaction with the matrix MOD_RES 2 2 N-acetylserine. MOD_RES 55 55 Phosphothreonine. MOD_RES 66 66 N6,N6,N6-trimethyllysine; alternate. MOD_RES 66 66 N6,N6-dimethyllysine; alternate. MOD_RES 66 66 N6-methyllysine; alternate. MOD_RES 197 197 N6,N6,N6-trimethyllysine; alternate. MOD_RES 197 197 N6,N6-dimethyllysine; alternate. MOD_RES 197 197 N6-methyllysine; alternate. MOD_RES 268 268 N6,N6,N6-trimethyllysine; alternate. MOD_RES 268 268 N6,N6-dimethyllysine; alternate. MOD_RES 268 268 N6-methyllysine; alternate. MOD_RES 284 284 N6,N6,N6-trimethyllysine; alternate. MOD_RES 284 284 N6,N6-dimethyllysine; alternate. MOD_RES 284 284 N6-methyllysine; alternate. |
Keyword | 3D-structure; Acetylation; Alternative initiation; Alternative splicing; Chromatin regulator; Chromosome; Complete proteome; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 441 AA |
Protein Sequence | MSEREVSTAP AGTDMPAAKK QKLSSDENSN PDLSGDENDD AVSIESGTNT ERPDTPTNTP 60 NAPGRKSWGK GKWKSKKCKY SFKCVNSLKE DHNQPLFGVQ FNWHSKEGDP LVFATVGSNR 120 VTLYECHSQG EIRLLQSYVD ADADENFYTC AWTYDSNTSH PLLAVAGSRG IIRIINPITM 180 QCIKHYVGHG NAINELKFHP RDPNLLLSVS KDHALRLWNI QTDTLVAIFG GVEGHRDEVL 240 SADYDLLGEK IMSCGMDHSL KLWRINSKRM MNAIKESYDY NPNKTNRPFI SQKIHFPDFS 300 TRDIHRNYVD CVRWLGDLIL SKSGRAILHS HQQCMRDPVS PNLRQHLSCE NAIVCWKPGK 360 MEDDIDKIKP SESNVTILGR FDYSQCDIWY MRFSMDFWQK MLALGNQVGK LYVWDLEVED 420 PHKAKCTTLT HHKCGAAIRQ TSFSRDSSIL IAVCDDASIW RWDRLR 466 |
Gene Ontology | GO:0035098; C:ESC/E(Z) complex; IDA:UniProtKB. GO:0045120; C:pronucleus; IEA:Compara. GO:0001739; C:sex chromatin; IEA:Compara. GO:0003682; F:chromatin binding; IEA:Compara. GO:0042054; F:histone methyltransferase activity; IDA:MGI. GO:0045892; P:negative regulation of transcription, DNA-dependent; NAS:UniProtKB. GO:0006349; P:regulation of gene expression by genetic imprinting; IEA:Compara. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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