CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005631
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hexokinase-2 
Protein Synonyms/Alias
 Hexokinase type II; HK II 
Gene Name
 Hk2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
398AAVLWRIKENKGEERacetylation[1]
418GVDGSVYKKHPHFAKacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 NP_BIND 84 89 ATP 1 (Potential).
 NP_BIND 425 426 ATP 1 (By similarity).
 NP_BIND 532 537 ATP 2 (By similarity).
 NP_BIND 747 748 ATP 2 (By similarity).
 NP_BIND 784 788 ATP 2 (By similarity).
 NP_BIND 863 867 ATP 2 (By similarity).
 REGION 1 12 Hydrophobic.
 REGION 13 475 Regulatory.
 REGION 84 88 Glucose-6-phosphate 1 binding (By
 REGION 155 156 Substrate 1 binding (By similarity).
 REGION 172 173 Substrate 1 binding (By similarity).
 REGION 208 209 Substrate 1 binding (By similarity).
 REGION 291 294 Substrate 1 binding (By similarity).
 REGION 413 415 Glucose-6-phosphate 1 binding (By
 REGION 476 917 Catalytic.
 REGION 532 536 Glucose-6-phosphate 2 binding (By
 REGION 603 604 Substrate 2 binding (By similarity).
 REGION 620 621 Substrate 2 binding (By similarity).
 REGION 656 657 Substrate 2 binding (By similarity).
 REGION 739 742 Substrate 2 binding (By similarity).
 REGION 861 863 Glucose-6-phosphate 2 binding (By
 BINDING 30 30 ATP 1 (By similarity).
 BINDING 209 209 Glucose-6-phosphate 1 (By similarity).
 BINDING 232 232 Glucose-6-phosphate 1 (By similarity).
 BINDING 235 235 Substrate 1 (By similarity).
 BINDING 260 260 Substrate 1 (By similarity).
 BINDING 449 449 Glucose-6-phosphate 1 (By similarity).
 BINDING 558 558 ATP 2 (Potential).
 BINDING 657 657 Glucose-6-phosphate 2 (By similarity).
 BINDING 680 680 ATP 2 (By similarity).
 BINDING 680 680 Glucose-6-phosphate 2 (By similarity).
 BINDING 683 683 Substrate 2 (By similarity).
 BINDING 708 708 Substrate 2 (By similarity).
 BINDING 897 897 Glucose-6-phosphate 2 (By similarity).
 MOD_RES 461 461 Phosphotyrosine (By similarity).  
Keyword
 Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 917 AA 
Protein Sequence
MIASHMIACL FTELNQNQVQ KVDQFLYHMR LSDETLLEIS RRFRKEMEKG LGATTHPTAA 60
VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN GLQRVEMENQ IYAIPEDIMR 120
GSGTQLFDHI AECLANFMDK LQIKEKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV 180
EGRDVVDLIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME 240
EMRHIDMVEG DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM 300
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEEDKDGIE KAYQILMRLG 360
LNPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN KGEERLRSTI GVDGSVYKKH 420
PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLESLKLS 480
HEQLLEVKRR MKVEMEQGLS KETHAVAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV 540
LLVRVRNGKR RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF 600
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG 660
TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE GRMCVNMEWG AFGDNGCLDD 720
LRTVFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG 780
IFETKFLSQI ESDCLALLQV RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA 840
VVDKIRENRG LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG 900
AALITAVACR IREAGQR 917 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005741; C:mitochondrial outer membrane; TAS:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0008865; F:fructokinase activity; IBA:RefGenome.
 GO:0004340; F:glucokinase activity; IBA:RefGenome.
 GO:0005536; F:glucose binding; IDA:RGD.
 GO:0019158; F:mannokinase activity; IBA:RefGenome.
 GO:0008637; P:apoptotic mitochondrial changes; IEA:Compara.
 GO:0001678; P:cellular glucose homeostasis; IBA:RefGenome.
 GO:0006096; P:glycolysis; IBA:RefGenome.
 GO:0007595; P:lactation; IEP:RGD.
 GO:0046324; P:regulation of glucose import; IEA:Compara. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.