UniProt Accession

 RUVB2_YEAST; Q12464; D6W3D5 

Genbank Protein ID

 Z67751; X94561; Z73591; BK006949 

Genbank Nucleotide ID

 CAA91609.1; CAA64252.1; CAA97952.1; DAA11201.1 

Protein Name

 RuvB-like protein 2 

Protein Synonyms/Alias

 RUVBL2; TIP49-homology protein 2; TIP49b homolog 

Gene Name

 RVB2 

Gene Synonyms/Alias

 TIH2; TIP49B; YPL235W; P1060 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
174	TIYELGNKMIDGLTK	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing. 

Sequence Annotation

 NP_BIND 75 82 ATP (By similarity).  

Keyword

 Activator; ATP-binding; Chromatin regulator; Complete proteome; DNA damage; DNA repair; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; rRNA processing; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 471 AA 

Protein Sequence

Gene Ontology

 GO:0070209; C:ASTRA complex; IDA:SGD.
 GO:0031011; C:Ino80 complex; IPI:SGD.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0097255; C:R2TP complex; IDA:SGD.
 GO:0000812; C:Swr1 complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IDA:SGD.
 GO:0043141; F:ATP-dependent 5'-3' DNA helicase activity; IDA:SGD.
 GO:0000492; P:box C/D snoRNP assembly; IMP:SGD.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0043486; P:histone exchange; IPI:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase.
 IPR027238; RuvB-like.
 IPR010339; TIP49_C. 

Pfam

 PF06068; TIP49 

SMART

 SM00382; AAA 

PROSITE

  

PRINTS