| Tag | Content |
|---|
| CPLM ID | CPLM-005493 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | APC/C activator protein CDC20 |
Protein Synonyms/Alias | Cell division control protein 20 |
Gene Name | CDC20 |
Gene Synonyms/Alias | YGL116W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 241 | QKIPDLIKLRKINTN | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle. |
Sequence Annotation | REPEAT 257 296 WD 1.
REPEAT 299 338 WD 2.
REPEAT 342 379 WD 3.
REPEAT 383 422 WD 4.
REPEAT 425 467 WD 5.
REPEAT 469 519 WD 6.
REPEAT 523 562 WD 7.
MOTIF 17 25 D-box.
MOTIF 85 101 Bipartite nuclear localization signal.
MOTIF 144 150 C-box.
MOTIF 586 592 KEN box (Potential). |
Keyword | Cell cycle; Cell division; Complete proteome; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 610 AA |
Protein Sequence | MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS SSLNIRNSKR 60
PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD KATFSAYSSR SYPTIGSESV 120
VSQTSLSQPT TSREVDEQFT VAADRYIPIL QGASQNKVDP ETLHEALPPP NASPISHLRA 180
QTKIVFKQNV AEACGLDMNK RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI 240
KLRKINTNPE RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE 300
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL SWLDTLIATG 360
SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL ASGGNDNTVM IWDTRTSLPQ 420
FSKKTHTAAV KALSWCPYSP NILASGGGQT DKHIHFWNSI TGARVGSINT GSQVSSLHWG 480
QSHTSTNGGM MNKEIVATGG NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL 540
ATVGGDENLK FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR 600
PSSTSQYLIR 610 |
Gene Ontology | GO:0005680; C:anaphase-promoting complex; IPI:SGD. GO:0005737; C:cytoplasm; IDA:SGD. GO:0033597; C:mitotic checkpoint complex; IDA:SGD. GO:0010997; F:anaphase-promoting complex binding; IDA:SGD. GO:0097027; F:ubiquitin-protein ligase activator activity; IDA:SGD. GO:0051487; P:activation of anaphase-promoting complex activity involved in meiotic cell cycle; IMP:SGD. GO:0007092; P:activation of mitotic anaphase-promoting complex activity; IMP:SGD. GO:0051301; P:cell division; IEA:UniProtKB-KW. GO:0008054; P:cyclin catabolic process; IMP:SGD. GO:0007067; P:mitosis; IEA:UniProtKB-KW. GO:0031577; P:spindle checkpoint; IPI:SGD. |
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PROSITE | |
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