CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019602
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein LAP2 
Protein Synonyms/Alias
 Densin-180-like protein; Erbb2-interacting protein; Erbin 
Gene Name
 ERBB2IP 
Gene Synonyms/Alias
 ERBIN; KIAA1225; LAP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
371MGDMQKLKVINLSDNubiquitination[1]
381NLSDNRLKNLPFSFTubiquitination[1]
404WLSDNQSKPLIPLQKubiquitination[1]
419ETDSETQKMVLTNYMubiquitination[1]
492TPYPDELKNMVKTVQubiquitination[2, 3]
496DELKNMVKTVQTIVHubiquitination[1]
506QTIVHRLKDEETNEDsumoylation[4]
519EDSGRDLKPHEDQQDubiquitination[5]
694THIDINSKIRQEDENubiquitination[2]
1023TENQSYAKHSANMNFubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state. 
Sequence Annotation
 REPEAT 23 44 LRR 1.
 REPEAT 47 68 LRR 2.
 REPEAT 70 91 LRR 3.
 REPEAT 93 114 LRR 4.
 REPEAT 116 137 LRR 5.
 REPEAT 139 161 LRR 6.
 REPEAT 162 183 LRR 7.
 REPEAT 185 206 LRR 8.
 REPEAT 208 229 LRR 9.
 REPEAT 231 252 LRR 10.
 REPEAT 254 275 LRR 11.
 REPEAT 277 298 LRR 12.
 REPEAT 300 321 LRR 13.
 REPEAT 323 344 LRR 14.
 REPEAT 346 367 LRR 15.
 REPEAT 369 391 LRR 16.
 REPEAT 392 413 LRR 17.
 DOMAIN 1321 1410 PDZ.
 MOD_RES 440 440 Phosphoserine.
 MOD_RES 569 569 Phosphoserine.
 MOD_RES 598 598 Phosphoserine (By similarity).
 MOD_RES 602 602 Phosphoserine.
 MOD_RES 603 603 Phosphoserine.
 MOD_RES 715 715 Phosphoserine (By similarity).
 MOD_RES 853 853 Phosphothreonine (By similarity).
 MOD_RES 857 857 Phosphoserine.
 MOD_RES 872 872 Phosphoserine.
 MOD_RES 917 917 Phosphothreonine.
 MOD_RES 920 920 Phosphotyrosine.
 MOD_RES 931 931 Phosphoserine.
 MOD_RES 972 972 Phosphotyrosine.
 MOD_RES 1104 1104 Phosphotyrosine (By similarity).
 MOD_RES 1158 1158 Phosphoserine.
 MOD_RES 1179 1179 Phosphoserine (By similarity).
 MOD_RES 1286 1286 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Complete proteome; Leucine-rich repeat; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1412 AA 
Protein Sequence
MTTKRSLFVR LVPCRCLRGE EETVTTLDYS HCSLEQVPKE IFTFEKTLEE LYLDANQIEE 60
LPKQLFNCQS LHKLSLPDND LTTLPASIAN LINLRELDVS KNGIQEFPEN IKNCKVLTIV 120
EASVNPISKL PDGFSQLLNL TQLYLNDAFL EFLPANFGRL TKLQILELRE NQLKMLPKTM 180
NRLTQLERLD LGSNEFTEVP EVLEQLSGLK EFWMDANRLT FIPGFIGSLK QLTYLDVSKN 240
NIEMVEEGIS TCENLQDLLL SSNSLQQLPE TIGSLKNITT LKIDENQLMY LPDSIGGLIS 300
VEELDCSFNE VEALPSSIGQ LTNLRTFAAD HNYLQQLPPE IGSWKNITVL FLHSNKLETL 360
PEEMGDMQKL KVINLSDNRL KNLPFSFTKL QQLTAMWLSD NQSKPLIPLQ KETDSETQKM 420
VLTNYMFPQQ PRTEDVMFIS DNESFNPSLW EEQRKQRAQV AFECDEDKDE REAPPREGNL 480
KRYPTPYPDE LKNMVKTVQT IVHRLKDEET NEDSGRDLKP HEDQQDINKD VGVKTSESTT 540
TVKSKVDERE KYMIGNSVQK ISEPEAEISP GSLPVTANMK ASENLKHIVN HDDVFEESEE 600
LSSDEEMKMA EMRPPLIETS INQPKVVALS NNKKDDTKET DSLSDEVTHN SNQNNSNCSS 660
PSRMSDSVSL NTDSSQDTSL CSPVKQTHID INSKIRQEDE NFNSLLQNGD ILNSSTEEKF 720
KAHDKKDFNL PEYDLNVEER LVLIEKSVDS TATADDTHKL DHINMNLNKL ITNDTFQPEI 780
MERSKTQDIV LGTSFLSINS KEETEHLENG NKYPNLESVN KVNGHSEETS QSPNRTEPHD 840
SDCSVDLGIS KSTEDLSPQK SGPVGSVVKS HSITNMEIGG LKIYDILSDN GPQQPSTTVK 900
ITSAVDGKNI VRSKSATLLY DQPLQVFTGS SSSSDLISGT KAIFKFDSNH NPEEPNIIRG 960
PTSGPQSAPQ IYGPPQYNIQ YSSSAAVKDT LWHSKQNPQI DHASFPPQLL PRSESTENQS 1020
YAKHSANMNF SNHNNVRANT AYHLHQRLGP ARHGEMWAIS PNDRLIPAVT RSTIQRQSSV 1080
SSTASVNLGD PGSTRRAQIP EGDYLSYREF HSAGRTPPMM PGSQRPLSAR TYSIDGPNAS 1140
RPQSARPSIN EIPERTMSVS DFNYSRTSPS KRPNARVGSE HSLLDPPGKS KVPRDWREQV 1200
LRHIEAKKLE KKHPQTSSSG DPCQDGIFIS GQQNYSSATL SHKDVPPDSL MKMPLSNGQM 1260
GQPLRPQANY SQIHHPPQAS VARHPSREQL IDYLMLKVAH QPPYTQPHCS PRQGHELAKQ 1320
EIRVRVEKDP ELGFSISGGV GGRGNPFRPD DDGIFVTRVQ PEGPASKLLQ PGDKIIQANG 1380
YSFINIEHGQ AVSLLKTFQN TVELIIVREV SS 1412 
Gene Ontology
 GO:0009925; C:basal plasma membrane; NAS:UniProtKB.
 GO:0005604; C:basement membrane; TAS:ProtInc.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030056; C:hemidesmosome; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005176; F:ErbB-2 class receptor binding; TAS:UniProtKB.
 GO:0005200; F:structural constituent of cytoskeleton; NAS:UniProtKB.
 GO:0045175; P:basal protein localization; NAS:UniProtKB.
 GO:0007155; P:cell adhesion; NAS:UniProtKB.
 GO:0007049; P:cell cycle; NAS:UniProtKB.
 GO:0016049; P:cell growth; NAS:UniProtKB.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:UniProtKB.
 GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; NAS:UniProtKB.
 GO:0007229; P:integrin-mediated signaling pathway; NAS:UniProtKB.
 GO:0045104; P:intermediate filament cytoskeleton organization; NAS:UniProtKB.
 GO:0006605; P:protein targeting; IEA:Compara. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR025875; Leu-rich_rpt_4.
 IPR001478; PDZ. 
Pfam
 PF12799; LRR_4
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS51450; LRR
 PS50106; PDZ 
PRINTS