UniProt Accession

 GLRX3_HUMAN; O76003; B3KMP7; B3KMQ5; D3DRG2; Q5JV01; Q96CE0; Q9P1B0; Q9P1B1 

Genbank Protein ID

 AF118649; AF118652; AJ010841; AK022131; AK021926; AL139123; CH471066; CH471066; CH471066; BC005289; BC014372 

Genbank Nucleotide ID

 AAF28841.1; AAF28844.1; CAA09375.1; BAG51067.1; BAG51059.1; CAC40691.1; EAW49152.1; EAW49154.1; EAW49155.1; AAH05289.1; AAH14372.2 

Protein Name

 Glutaredoxin-3 

Protein Synonyms/Alias

 PKC-interacting cousin of thioredoxin; PICOT; PKC-theta-interacting protein; PKCq-interacting protein; Thioredoxin-like protein 2 

Gene Name

 GLRX3 

Gene Synonyms/Alias

 PICOT; TXNL2; HUSSY-22 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
67	LPQVSFVKLEAEGVP	ubiquitination	[1]
79	GVPEVSEKYEISSVP	ubiquitination	[1]
92	VPTFLFFKNSQKIDR	acetylation	[2]
92	VPTFLFFKNSQKIDR	ubiquitination	[1, 3, 4]
110	AHAPELTKKVQRHAS	acetylation	[5, 6]
111	HAPELTKKVQRHASS	ubiquitination	[7]
139	DLNLRLKKLTHAAPC	ubiquitination	[7]
163	EPRCGFSKQMVEILH	ubiquitination	[6, 7, 8]
240	PKLEERLKVLTNKAS	ubiquitination	[3, 4, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity. 

Sequence Annotation

 DOMAIN 2 117 Thioredoxin.
 DOMAIN 144 236 Glutaredoxin 1.
 DOMAIN 237 335 Glutaredoxin 2.
 METAL 159 159 Iron-sulfur (2Fe-2S); shared with dimeric
 METAL 261 261 Iron-sulfur (2Fe-2S); shared with dimeric
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0030018; C:Z disc; IEA:UniProtKB-SubCell.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0010614; P:negative regulation of cardiac muscle hypertrophy; ISS:UniProtKB.
 GO:0002026; P:regulation of the force of heart contraction; ISS:UniProtKB. 

Interpro

 IPR002109; Glutaredoxin.
 IPR004480; Monothiol_GRX-rel.
 IPR012336; Thioredoxin-like_fold.
 IPR013766; Thioredoxin_domain. 

Pfam

 PF00462; Glutaredoxin
 PF00085; Thioredoxin 

SMART

  

PROSITE

 PS51354; GLUTAREDOXIN_2
 PS00194; THIOREDOXIN_1
 PS51352; THIOREDOXIN_2 

PRINTS