CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028571
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Zinc finger protein RFP 
Protein Synonyms/Alias
  
Gene Name
 TRIM27 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*LKQFTEKMQSDMEKubiquitination[1]
114WEVEVGDKAKWTIGVubiquitination[1, 2, 3, 4, 5]
116VEVGDKAKWTIGVCEubiquitination[1, 2, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 247 AA 
Protein Sequence
LKQFTEKMQS DMEKIQESAD SLEGGILDLG SVLSHILPVS ELREAQLYSV DVTLDPDTAY 60
PSLILSDNLR QVRYSYLQQD LPDNPERFNL FPCVLGSPCF IAGRHYWEVE VGDKAKWTIG 120
VCEDSVCRKG GVTSAPQNGF WAVSLWYGKE YWALTSPMTA LPLRTPLQRV GIFLDYDAGE 180
VSFYNVTERC HTFTFSHATF CGPVRPYFSL SYSGGKSAAP LIICPMSGID GFSGHVGNHG 240
HSMETSP 247 
Gene Ontology
  
Interpro
 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR008985; ConA-like_lec_gl_sf.
 IPR006574; PRY.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF00622; SPRY 
SMART
 SM00589; PRY
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY 
PRINTS
 PR01407; BUTYPHLNCDUF.