CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ceramide glucosyltransferase 
Protein Synonyms/Alias
 GLCT-1; Glucosylceramide synthase; GCS; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase 
Gene Name
 UGCG 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44LNKKATDKQPYSKLPubiquitination[1, 2, 3]
49TDKQPYSKLPGVSLLubiquitination[1, 2, 3]
57LPGVSLLKPLKGVDPubiquitination[1, 2, 3]
104VCKKLLGKYPNVDARubiquitination[1, 3, 4]
124KKVGINPKINNLMPGubiquitination[1, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase". 
Sequence Annotation
  
Keyword
 Complete proteome; Glycosyltransferase; Golgi apparatus; Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome; Sphingolipid metabolism; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 394 AA 
Protein Sequence
MALLDLALEG MAVFGFVLFL VLWLMHFMAI IYTRLHLNKK ATDKQPYSKL PGVSLLKPLK 60
GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK LLGKYPNVDA RLFIGGKKVG 120
INPKINNLMP GYEVAKYDLI WICDSGIRVI PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF 180
AATLEQVYFG TSHPRYYISA NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA 240
KAIADRGWRF AMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS 300
LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA VAWFIRESMT 360
IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV 394 
Gene Ontology
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0008120; F:ceramide glucosyltransferase activity; TAS:ProtInc.
 GO:0008544; P:epidermis development; TAS:ProtInc.
 GO:0006679; P:glucosylceramide biosynthetic process; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR025993; Ceramide_glucosylTrfase. 
Pfam
 PF13506; Glyco_transf_21 
SMART
  
PROSITE
  
PRINTS