CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024179
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial carnitine/acylcarnitine carrier protein 
Protein Synonyms/Alias
 Carnitine/acylcarnitine translocase; CAC; mCAC; Solute carrier family 25 member 20 
Gene Name
 Slc25a20 
Gene Synonyms/Alias
 Cac; Cact 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
6**MADEPKPISPFKNacetylation[1]
148QASSGENKYSGTLDCacetylation[1, 2, 3]
157SGTLDCAKKLYQEFGacetylation[3]
158GTLDCAKKLYQEFGIacetylation[3]
170FGIRGFYKGTVLTLMacetylation[3, 4]
170FGIRGFYKGTVLTLMsuccinylation[4]
202NLFTPEGKSVSDLSVubiquitination[5]
244FQTAPPGKYPNGFRDacetylation[1, 2, 6]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway (By similarity). 
Sequence Annotation
 REPEAT 8 99 Solcar 1.
 REPEAT 108 196 Solcar 2.
 REPEAT 207 293 Solcar 3.
 MOD_RES 148 148 N6-acetyllysine.
 MOD_RES 244 244 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 301 AA 
Protein Sequence
MADEPKPISP FKNLLAGGFG GMCLVFVGHP LDTVKVRLQT QPPSLSGQPP MYSGTLDCFR 60
KTLMREGITG LYRGMAAPII GVTPMFAVCF FGFGLGKKLQ QKSPEDELSY PQLFTAGMLS 120
GVFTTGIMTP GERIKCLLQI QASSGENKYS GTLDCAKKLY QEFGIRGFYK GTVLTLMRDV 180
PASGMYFMTY EWLKNLFTPE GKSVSDLSVP RILVAGGFAG IFNWAVAIPP DVLKSRFQTA 240
PPGKYPNGFR DVLRELIREE GVTSLYKGFN AVMIRAFPAN AACFLGFEIA MKFLNWIAPN 300
L 301 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 
Pfam
 PF00153; Mito_carr 
SMART
  
PROSITE
 PS50920; SOLCAR 
PRINTS