CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-tubulin complex component 2 
Protein Synonyms/Alias
 GCP-2; hGCP2; Gamma-ring complex protein 103 kDa; h103p; hGrip103; Spindle pole body protein Spc97 homolog; hSpc97 
Gene Name
 TUBGCP2 
Gene Synonyms/Alias
 GCP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92LSKLTEDKETLQYLQubiquitination[1]
157LQQSLELKRKMLRDKubiquitination[1, 2]
426IQEDYNDKYWDQRYTubiquitination[2]
445QIPSFLQKMADKILSubiquitination[2]
455DKILSTGKYLNVVREubiquitination[3]
595RVLAIETKQEKAMAHubiquitination[2]
827ATINKFDKNFSAHLLacetylation[4]
879LSAERSQKATPQVPVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. 
Sequence Annotation
 MOD_RES 83 83 Phosphotyrosine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 902 AA 
Protein Sequence
MSEFRIHHDV NELLSLLRVH GGDGAEVYID LLQKNRTPYV TTTVSAHSAK VKIAEFSRTP 60
EDFLKKYDEL KSKNTRNLDP LVYLLSKLTE DKETLQYLQQ NAKERAELAA AAVGSSTTSI 120
NVPAAASKIS MQELEELRKQ LGSVATGSTL QQSLELKRKM LRDKQNKKNS GQHLPIFPAW 180
VYERPALIGD FLIGAGISTD TALPIGTLPL ASQESAVVED LLYVLVGVDG RYVSAQPLAG 240
RQSRTFLVDP NLDLSIRELV HRILPVAASY SAVTRFIEEK SSFEYGQVNH ALAAAMRTLV 300
KEHLILVSQL EQLHRQGLLS LQKLWFYIQP AMRTMDILAS LATSVDKGEC LGGSTLSLLH 360
DRSFSYTGDS QAQELCLYLT KAASAPYFEV LEKWIYRGII HDPYSEFMVE EHELRKERIQ 420
EDYNDKYWDQ RYTIVQQQIP SFLQKMADKI LSTGKYLNVV RECGHDVTCP VAKEIIYTLK 480
ERAYVEQIEK AFNYASKVLL DFLMEEKELV AHLRSIKRYF LMDQGDFFVH FMDLAEEELR 540
KPVEDITPPR LEALLELALR MSTANTDPFK DDLKIDLMPH DLITQLLRVL AIETKQEKAM 600
AHADPTELAL SGLEAFSFDY IVKWPLSLII NRKALTRYQM LFRHMFYCKH VERQLCSVWI 660
SNKTAKQHSL HSAQWFAGAF TLRQRMLNFV QNIQYYMMFE VMEPTWHILE KNLKSASNID 720
DVLGHHTGFL DTCLKDCMLT NPELLKVFSK LMSVCVMFTN CMQKFTQSMK LDGELGGQTL 780
EHSTVLGLPA GAEERARKEL ARKHLAEHAD TVQLVSGFEA TINKFDKNFS AHLLDLLARL 840
SIYSTSDCEH GMASVISRLD FNGFYTERLE RLSAERSQKA TPQVPVLRGP PAPAPRVAVT 900
AQ 902 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005881; C:cytoplasmic microtubule; TAS:ProtInc.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000922; C:spindle pole; IEA:InterPro.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007020; P:microtubule nucleation; TAS:ProtInc.
 GO:0006461; P:protein complex assembly; TAS:ProtInc. 
Interpro
 IPR007259; Spc97_Spc98. 
Pfam
 PF04130; Spc97_Spc98 
SMART
  
PROSITE
  
PRINTS