CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023033
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 21 
Protein Synonyms/Alias
  
Gene Name
 KLHL21 
Gene Synonyms/Alias
 KIAA0469 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33SQLRAERKFLDVTLEubiquitination[1]
194DDGLCVPKEEAAYQLubiquitination[1]
370AEVAPMLKAREYHSSubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
494PTRNEWDKIPSMNQVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. 
Sequence Annotation
 DOMAIN 35 103 BTB.
 DOMAIN 138 239 BACK.
 REPEAT 287 335 Kelch 1.
 REPEAT 336 382 Kelch 2.
 REPEAT 384 422 Kelch 3.
 REPEAT 423 470 Kelch 4.
 REPEAT 472 512 Kelch 5.
 REPEAT 513 560 Kelch 6.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kelch repeat; Mitosis; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY 60
FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA 120
VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR 180
LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAAHWPQL LEAVRLPFVR RFYLLAHVEA 240
EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE 300
LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN 360
EWAEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHTTD SWEALQPMTY PMDNCSTTAC 420
RGRLYAIGSL AGKETMVMQC YDPDTDLWSL VDCGQLPPWS FAPKTATLNG LMYFVRDDSA 480
EVDVYNPTRN EWDKIPSMNQ VHVGGSLAVL GGKLYVSGGY DNTFELSDVV EAYDPETRAW 540
SVVGRLPEPT FWHGSVSIFR QFMPQTFSGG RGFELDSGSD DMDPGRPRPP RDPDELH 597 
Gene Ontology
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005827; C:polar microtubule; IDA:UniProtKB.
 GO:0051322; P:anaphase; IMP:UniProtKB.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS