CPLM-004471

Genbank Nucleotide ID

Pyruvate decarboxylase isozyme 2

Protein Synonyms/Alias

YLR134W; L3133; L9606.7

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
41	LDKLYEVKGMRWAGN	acetylation	[1]
190	TPIDLSLKPNDAEAE	acetylation	[2]

[1] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
Xie H, Bandhakavi S, Roe MR, Griffin TJ.
J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211]
[2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

Second most abundant of three pyruvate decarboxylases (PDC1, PDC5, PDC6) implicated in the nonoxidative conversion of pyruvate to acetaldehyde and carbon dioxide during alcoholic fermentation. Most of the produced acetaldehyde is subsequently reduced to ethanol, but some is required for cytosolic acetyl-CoA production for biosynthetic pathways. The enzyme is also one of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) able to decarboxylate more complex 2-oxo acids (alpha-keto-acids) than pyruvate, which seem mainly involved in amino acid catabolism. Here the enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids. In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids valine, isoleucine, phenylalanine, and tryptophan, whereas leucine is no substrate. In a side-reaction the carbanionic intermediate (or active aldehyde) generated by decarboxylation or by activation of an aldehyde can react with an aldehyde via condensation (or carboligation) yielding a 2-hydroxy ketone, collectively called acyloins.

REGION 390 476 Thiamine pyrophosphate binding (By
METAL 444 444 Magnesium (By similarity).
METAL 471 471 Magnesium (By similarity).
METAL 473 473 Magnesium; via carbonyl oxygen (By
BINDING 28 28 Substrate (By similarity).
BINDING 115 115 Substrate (By similarity).
BINDING 157 157 Substrate; allosteric site (By
BINDING 477 477 Substrate (By similarity).

Allosteric enzyme; Branched-chain amino acid catabolism; Complete proteome; Cytoplasm; Decarboxylase; Lyase; Magnesium; Metal-binding; Nucleus; Phenylalanine catabolism; Reference proteome; Thiamine pyrophosphate; Tryptophan catabolism.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:SGD.
GO:0005634; C:nucleus; IDA:SGD.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0004737; F:pyruvate decarboxylase activity; IDA:SGD.
GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
GO:0000949; P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway; IGI:SGD.
GO:0009083; P:branched-chain amino acid catabolic process; IEA:UniProtKB-KW.
GO:0019655; P:glucose catabolic process to ethanol; IDA:SGD.
GO:0006559; P:L-phenylalanine catabolic process; IGI:SGD.
GO:0006090; P:pyruvate metabolic process; IDA:SGD.
GO:0006569; P:tryptophan catabolic process; IGI:SGD.

IPR012000; Thiamin_PyroP_enz_cen_dom.
IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399; TPP-bd_CS.
IPR012110; TPP_enzyme.
IPR011766; TPP_enzyme-bd_C.

PF02775; TPP_enzyme_C
PF00205; TPP_enzyme_M
PF02776; TPP_enzyme_N

PS00187; TPP_ENZYMES