CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040857
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mediator of RNA polymerase II transcription subunit 12-like protein 
Protein Synonyms/Alias
  
Gene Name
 MED12L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82SILAEKLKLNTFQDTubiquitination[1]
292YEVEQQIKQRGRAVEubiquitination[1]
306EVRWSFDKCQESTAGubiquitination[1]
390HRAMAVAKLLEKRQAubiquitination[1]
595ILLYGVGKERDEARHubiquitination[1]
606EARHQLKKITKDILKubiquitination[1]
609HQLKKITKDILKILNubiquitination[1]
631GVGDEGQKARKNKQEubiquitination[1]
818SSACSKVKQTIYNNVubiquitination[1]
858INYSMLGKILSDNAAubiquitination[1]
1010FLPQATGKPFPGIRSubiquitination[1, 2]
1060NNSVSSLKNDDFTMRubiquitination[1, 2]
1090QNPKSCGKSISIETAubiquitination[1]
1158ICYPHGIKECTEGDNubiquitination[1]
1291SVQGRVLKAAGEELEubiquitination[1]
1317ERDRQKQKSMSLLSQubiquitination[1]
1370ERYQDDIKARQMMHEubiquitination[1, 2]
1510LIDTKGNKIAGFDSIubiquitination[1]
1520GFDSIDKKQGLQVSTubiquitination[1]
1530LQVSTKQKVSPWDLFubiquitination[1]
1711SFVPTNTKQALSNMLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1809 AA 
Protein Sequence
MAAFGLLSYE QRPLKRPRLG PPDVYPQDPK QKEDELTAVN VKQGFNNQPA FTGDEHGSAR 60
NIVINPSKIG AYFSSILAEK LKLNTFQDTG KKKPQVNAKD NYWLVTARSQ SAIHSWFSDL 120
AGNKPLSILA KKVPILSKKE DVFAYLAKYS VPMVRATWLI KMTCAYYSAI SEAKIKKRQA 180
PDPNLEWTQI STRYLREQLA KISDFYHMAS STGDGPVPVP PEVEQAMKQW EYNEKLAFHM 240
FQEGMLEKHE YLTWILDVLE KIRPMDDDLL KLLLPLMLQV RARIYEVEQQ IKQRGRAVEV 300
RWSFDKCQES TAGVTISRVL HTLEVLDRHC FDRTDSSNSM ETLYHKIFWA NQNKDNQEVA 360
PNDEAVVTLL CEWAVSCKRS GKHRAMAVAK LLEKRQAEIE AERCGESEVL DEKESISSSS 420
LAGSSLPVFQ NVLLRFLDTQ APSLSDPNSE CEKVEFVNLV LLFCEFIRHD VFSHDAYMCT 480
LISRGDLSVT ASTRPRSPVG ENADEHYSKD HDVKMEIFSP MPGESCENAN TSLGRRMSVN 540
CEKLVKREKP RELIFPSNYD LLRHLQYATH FPIPLDESSS HECNQRTILL YGVGKERDEA 600
RHQLKKITKD ILKILNKKST TETGVGDEGQ KARKNKQETF PTLETVFTKL QLLSYFDQHQ 660
VTSQISNNVL EQITSFASGT SYHLPLAHHI QLIFDLMEPA LNINGLIDFA IQLLNELSVV 720
EAELLLKSSS LAGSYTTGLC VCIVAVLRRY HSCLILNPDQ TAQVFEGLCG VVKHVVNPSE 780
CSSPERCILA YLYDLYVSCS HLRSKFGDLF SSACSKVKQT IYNNVMPANS NLRWDPDFMM 840
DFIENPSARS INYSMLGKIL SDNAANRYSF VCNTLMNVCM GHQDAGRIND IANFSSELTA 900
CCTVLSSEWL GVLKALCCSS NHVWGFNDVL CTVDVSDLSF HDSLATFIAI LIARQCFSLE 960
DVVQHVALPS LLAAACGDAD AEPGARMTCR LLLHLFRAPQ ACFLPQATGK PFPGIRSSCD 1020
RHLLAAAHNS IEVGAVFAVL KAIMMLGDAK IGNNSVSSLK NDDFTMRGLR CDGNADDIWT 1080
ASQNPKSCGK SISIETANLR EYARYVLRTI CQQEWVGEHC LKEPERLCTD KELILDPVLS 1140
NMQAQKLLQL ICYPHGIKEC TEGDNLQRQH IKRILQNLEQ WTLRQSWLEL QLMIKQCLKD 1200
PGSGSVAEMN NLLDNIAKAT IEVFQQSADL NNSSNSGMSL FNPNSIGSAD TSSTRQNGIK 1260
TFLSSSERRG VWLVAPLIAR LPTSVQGRVL KAAGEELEKG QHLGSSSKKE RDRQKQKSMS 1320
LLSQQPFLSL VLTCLKGQDE QREGLLTSLQ NQVNQILSNW REERYQDDIK ARQMMHEALQ 1380
LRLNLVGGMF DTVQRSTQWT TDWALLLLQI ITSGTVDMHT NNELFTTVLD MLGVLINGTL 1440
ASDLSNASPG GSEENKRAYM NLVKKLKKEL GDKRSESIDK VRQLLPLPKQ TCDVITCEPM 1500
GSLIDTKGNK IAGFDSIDKK QGLQVSTKQK VSPWDLFEGQ KNPAPLSWAW FGTVRVDRRV 1560
IKYEEQHHLL LYHTHPMPKP RSYYLQPLPL PPEEEEEEPT SPVSQEPERK SAELSDQGKT 1620
TTDEEKKTKG RKRKTKSSSR VDEYPQSNIY RVPPNYSPIS SQMMHHPQST LWGYNLVGQP 1680
QQPGFFLQNQ SLTPGGSRLD PAGSFVPTNT KQALSNMLQR RSGAMMQPPS LHAITSQQQL 1740
IQMKLLQQQQ QQRLLRQAQT RPFQQFPRQG LQQTQQQQQT AALVRQLQKQ LSSNQPQQGV 1800
TPYGHPSHF 1809 
Gene Ontology
 GO:0016592; C:mediator complex; IEA:InterPro.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 
Interpro
 IPR019035; Mediator_Med12.
 IPR021990; Mediator_Med12_LCEWAV. 
Pfam
 PF09497; Med12
 PF12145; Med12-LCEWAV 
SMART
  
PROSITE
  
PRINTS