UniProt Accession

 H2B2E_HUMAN; Q16778; A3KMC7; A8K110; Q4KMY1; Q5QNX0; Q9UE88 

Genbank Protein ID

 X57985; AY131979; CR541895; AK289725; AL591493; CH471121; BC005827; BC069193; BC096121; BC098112; BC098289; BC107084; BC107085; M60756 

Genbank Nucleotide ID

 CAA41051.1; AAN59961.1; CAG46693.1; BAF82414.1; CAI12568.1; EAW53605.1; AAH05827.1; AAH69193.1; AAH96121.1; AAH98112.1; AAH98289.1; AAI07085.1; AAI07086.1; AAA63192.1 

Protein Name

 Histone H2B type 2-E 

Protein Synonyms/Alias

 Histone H2B-GL105; Histone H2B.q; H2B/q 

Gene Name

 HIST2H2BE 

Gene Synonyms/Alias

 H2BFQ 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MPEPAKSAPAPKK	acetylation	[1, 2]
12	AKSAPAPKKGSKKAV	acetylation	[2]
12	AKSAPAPKKGSKKAV	glycation	[3]
13	KSAPAPKKGSKKAVT	acetylation	[1, 2]
13	KSAPAPKKGSKKAVT	glycation	[3]
16	PAPKKGSKKAVTKAQ	acetylation	[1, 2]
16	PAPKKGSKKAVTKAQ	glycation	[3]
17	APKKGSKKAVTKAQK	acetylation	[2]
21	GSKKAVTKAQKKDGK	acetylation	[1, 2]
24	KAVTKAQKKDGKKRK	acetylation	[2]
35	KKRKRSRKESYSIYV	glycation	[3]
47	IYVYKVLKQVHPDTG	methylation	[4]
47	IYVYKVLKQVHPDTG	ubiquitination	[5]
58	PDTGISSKAMGIMNS	methylation	[4]
58	PDTGISSKAMGIMNS	ubiquitination	[5]
86	SRLAHYNKRSTITSR	ubiquitination	[5]
109	LLPGELAKHAVSEGT	glycation	[3]
109	LLPGELAKHAVSEGT	methylation	[4]
109	LLPGELAKHAVSEGT	ubiquitination	[5]
117	HAVSEGTKAVTKYTS	glycation	[3]
117	HAVSEGTKAVTKYTS	ubiquitination	[5]
121	EGTKAVTKYTSSK**	ubiquitination	[5]

Reference

 [1] Overlapping but distinct patterns of histone acetylation by the human coactivators p300 and PCAF within nucleosomal substrates.
 Schiltz RL, Mizzen CA, Vassilev A, Cook RG, Allis CD, Nakatani Y.
 J Biol Chem. 1999 Jan 15;274(3):1189-92. [PMID: 9880483]
 [2] Proteome-wide prediction of acetylation substrates.
 Basu A, Rose KL, Zhang J, Beavis RC, Ueberheide B, Garcia BA, Chait B, Zhao Y, Hunt DF, Segal E, Allis CD, Hake SB.
 Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13785-90. [PMID: 19666589]
 [3] Glycation and oxidation of histones H2B and H1: in vitro study and characterization by mass spectrometry.
 Guedes S, Vitorino R, Domingues MR, Amado F, Domingues P.
 Anal Bioanal Chem. 2011 Apr;399(10):3529-39. [PMID: 21274518]
 [4] Update on activities at the Universal Protein Resource (UniProt) in 2013.
 e="String">UniProt Consortium.
 Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. 

Sequence Annotation

 MOD_RES 2 2 N-acetylproline (By similarity).
 MOD_RES 6 6 N6-acetyllysine; alternate.
 MOD_RES 6 6 N6-crotonyl-L-lysine; alternate.
 MOD_RES 12 12 N6-acetyllysine; alternate.
 MOD_RES 12 12 N6-crotonyl-L-lysine; alternate.
 MOD_RES 13 13 N6-acetyllysine; alternate.
 MOD_RES 13 13 N6-crotonyl-L-lysine; alternate.
 MOD_RES 15 15 Phosphoserine; by STK4/MST1.
 MOD_RES 16 16 N6-acetyllysine; alternate.
 MOD_RES 16 16 N6-crotonyl-L-lysine; alternate.
 MOD_RES 17 17 N6-acetyllysine; alternate.
 MOD_RES 17 17 N6-crotonyl-L-lysine; alternate.
 MOD_RES 21 21 N6-acetyllysine; alternate.
 MOD_RES 21 21 N6-crotonyl-L-lysine; alternate.
 MOD_RES 24 24 N6-acetyllysine; alternate (By
 MOD_RES 24 24 N6-crotonyl-L-lysine; alternate.
 MOD_RES 35 35 N6-crotonyl-L-lysine; alternate.
 MOD_RES 37 37 Phosphoserine; by AMPK (By similarity).
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 47 47 N6-methyllysine.
 MOD_RES 58 58 N6,N6-dimethyllysine.
 MOD_RES 109 109 N6-methyllysine.
 MOD_RES 113 113 Phosphoserine; alternate (By similarity).
 CARBOHYD 113 113 O-linked (GlcNAc...); alternate (By
 CROSSLNK 35 35 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 121 121 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Antibiotic; Antimicrobial; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Glycoprotein; Isopeptide bond; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 126 AA 

Protein Sequence

Gene Ontology

 GO:0000786; C:nucleosome; NAS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
 GO:0006334; P:nucleosome assembly; NAS:UniProtKB. 

Interpro

 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR000558; Histone_H2B. 

Pfam

 PF00125; Histone 

SMART

 SM00427; H2B 

PROSITE

 PS00357; HISTONE_H2B 

PRINTS

 PR00621; HISTONEH2B.