UniProt Accession

 TRMB_YEAST; Q12009; D6VRF3 

Genbank Protein ID

 X99000; Z74249; BK006938 

Genbank Nucleotide ID

 CAA67468.1; CAA98779.1; DAA11663.1 

Protein Name

 tRNA (guanine-N(7)-)-methyltransferase 

Protein Synonyms/Alias

 Transfer RNA methyltransferase 8; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase 

Gene Name

 TRM8 

Gene Synonyms/Alias

 YDL201W; D1075 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
32	RKELKHVKINESSLV	acetylation	[1]
149	LRNNTASKHGFQNIN	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Methyltransferase that catalyzes the formation of N(7)- methylguanine at position 46 (m7G46) in tRNA, a modification required to maintain stability of tRNAs; its absence resulting in tRNA decay. Both the D-stem and T-stem structures of tRNAs are required for efficient methyltransferase activity. 

Sequence Annotation

 REGION 126 127 S-adenosyl-L-methionine binding.
 REGION 161 162 S-adenosyl-L-methionine binding.
 REGION 259 261 S-adenosyl-L-methionine binding.
 ACT_SITE 184 184 Probable.
 BINDING 103 103 S-adenosyl-L-methionine; via carbonyl
 BINDING 181 181 S-adenosyl-L-methionine; via carbonyl
 MOD_RES 7 7 Phosphoserine; by ATM or ATR.
 MOD_RES 59 59 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 286 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0043527; C:tRNA methyltransferase complex; IDA:SGD.
 GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0036265; P:RNA (guanine-N7)-methylation; IDA:GOC.
 GO:0030488; P:tRNA methylation; IDA:SGD. 

Interpro

 IPR003358; tRNA_(Gua-N-7)_MeTrfase.
 IPR025763; tRNA_(Gua-N-7)_MeTrfase_euk. 

Pfam

 PF02390; Methyltransf_4 

SMART

  

PROSITE

  

PRINTS