UniProt Accession

 TBB4A_HUMAN; P04350; B3KQP4; Q969E5 

Genbank Protein ID

 X00734; AK075307; CH471139; BC006570; BC013683 

Genbank Nucleotide ID

 CAA25318.1; BAG52106.1; EAW69078.1; AAH06570.1; AAH13683.1 

Protein Name

 Tubulin beta-4A chain 

Protein Synonyms/Alias

 Tubulin 5 beta; Tubulin beta-4 chain 

Gene Name

 TUBB4A 

Gene Synonyms/Alias

 TUBB4; TUBB5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
252	QLNADLRKLAVNMVP	ubiquitination	[1]
297	TQQMFDAKNMMAACD	ubiquitination	[2]
324	FRGRMSMKEVDEQML	ubiquitination	[3, 4]
336	QMLSVQSKNSSYFVE	ubiquitination	[3, 4]
350	EWIPNNVKTAVCDIP	ubiquitination	[2]
362	DIPPRGLKMAATFIG	ubiquitination	[3, 4]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 

Sequence Annotation

 NP_BIND 140 146 GTP (Potential).
 MOD_RES 36 36 Phosphotyrosine (By similarity).
 MOD_RES 172 172 Phosphoserine; by CDK1 (By similarity).
 MOD_RES 366 366 Phosphothreonine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Microtubule; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 444 AA 

Protein Sequence

Gene Ontology

 GO:0005929; C:cilium; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0033269; C:internode region of axon; IEA:Compara.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0043209; C:myelin sheath; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
 GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007017; P:microtubule-based process; IEA:InterPro.
 GO:0051258; P:protein polymerization; IEA:InterPro. 

Interpro

 IPR013838; Beta-tubulin_BS.
 IPR002453; Beta_tubulin.
 IPR008280; Tub_FtsZ_C.
 IPR000217; Tubulin.
 IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
 IPR023123; Tubulin_C.
 IPR017975; Tubulin_CS.
 IPR003008; Tubulin_FtsZ_GTPase. 

Pfam

 PF00091; Tubulin
 PF03953; Tubulin_C 

SMART

 SM00864; Tubulin
 SM00865; Tubulin_C 

PROSITE

 PS00227; TUBULIN
 PS00228; TUBULIN_B_AUTOREG 

PRINTS

 PR01163; BETATUBULIN.
 PR01161; TUBULIN.