UniProt Accession

 SAT1_HUMAN; P21673 

Genbank Protein ID

 M77693; M55580; Z14136; U40369; BT006825; BC002503; BC008424 

Genbank Nucleotide ID

 AAA60573.1; AAA63260.1; CAA78509.1; AAA98854.1; AAP35471.1; AAH02503.1; AAH08424.1 

Protein Name

 Diamine acetyltransferase 1 

Protein Synonyms/Alias

 Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; Spermidine/spermine N(1)-acetyltransferase 1; SSAT; SSAT-1 

Gene Name

 SAT1 

Gene Synonyms/Alias

 SAT 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKFVIRPAT	ubiquitination	[1, 2]
26	RLIKELAKYEYMEEQ	acetylation	[3]
111	GIGSEILKNLSQVAM	ubiquitination	[4, 5]
158	EEGWRLFKIDKEYLL	ubiquitination	[1, 2, 4, 5]
161	WRLFKIDKEYLLKMA	ubiquitination	[1, 2, 4, 5]
166	IDKEYLLKMATEE**	ubiquitination	[4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. 

Sequence Annotation

 DOMAIN 4 171 N-acetyltransferase.
 REGION 27 28 Substrate binding.
 REGION 94 96 Acetyl-CoA binding.
 REGION 102 107 Acetyl-CoA binding.
 REGION 126 128 Substrate binding.
 REGION 133 136 Acetyl-CoA binding.
 REGION 140 143 Acetyl-CoA binding.
 BINDING 92 92 Substrate; via carbonyl oxygen.
 BINDING 152 152 Substrate.  

Keyword

 3D-structure; Acyltransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 171 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004145; F:diamine N-acetyltransferase activity; TAS:ProtInc.
 GO:0019809; F:spermidine binding; IEA:Compara.
 GO:0001525; P:angiogenesis; IEP:UniProtKB.
 GO:0006596; P:polyamine biosynthetic process; TAS:Reactome.
 GO:0009447; P:putrescine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR000182; GNAT_dom. 

Pfam

 PF00583; Acetyltransf_1 

SMART

  

PROSITE

 PS51186; GNAT 

PRINTS