| Tag | Content |
|---|
| CPLM ID | CPLM-021221 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 44 |
Protein Synonyms/Alias | Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44 |
Gene Name | USP44 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 106 | RRTLSAIKSQNYHCT | ubiquitination | [1] | | 244 | PASPAKDKVLSTSEN | ubiquitination | [1] | | 256 | SENEISQKVSDSSVK | ubiquitination | [1] | | 508 | ERYQCSGKDIASQPC | ubiquitination | [1] | | 543 | VCDQCNSKRRRFSSK | ubiquitination | [1] | | 550 | KRRRFSSKPVVLTEA | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. |
Sequence Annotation | ZN_FING 27 88 UBP-type.
ACT_SITE 282 282 Nucleophile.
ACT_SITE 636 636 Proton acceptor (By similarity).
MOD_RES 169 169 Phosphoserine (Probable).
MOD_RES 239 239 Phosphoserine (Probable).
MOD_RES 269 269 Phosphothreonine (Probable).
MOD_RES 401 401 Phosphoserine (Probable). |
Keyword | Cell cycle; Cell division; Complete proteome; Hydrolase; Metal-binding; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 712 AA |
Protein Sequence | MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH 60
FQESSHPVAL EVNEMYVFCY LCDDYVLNDN TTGDLKLLRR TLSAIKSQNY HCTTRSGRFL 120
RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL WHRRRILMGK IFRTWFEQSP IGRKKQEEPF 180
QEKIVVKREV KKRRQELEYQ VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP 240
AKDKVLSTSE NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LSHLLIFRQC 300
FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS SLSSGLSGGA 360
SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV SPFAMLHSVW RLIPAFRGYA 420
QQDAQEFLCE LLDKIQRELE TTGTSLPALI PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL 480
ACDNKSNTIE PFWDLSLEFP ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC 540
NSKRRRFSSK PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM 600
EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV HCNDSKLSMC 660
TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN EDADTSSNEI LS 712 |
Gene Ontology | GO:0005634; C:nucleus; IDA:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; IMP:UniProtKB. GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0051322; P:anaphase; IMP:UniProtKB. GO:0051301; P:cell division; IEA:UniProtKB-KW. GO:0007067; P:mitosis; IEA:UniProtKB-KW. GO:0060564; P:negative regulation of mitotic anaphase-promoting complex activity; IMP:UniProtKB. GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB. GO:0016579; P:protein deubiquitination; IDA:UniProtKB. GO:0090266; P:regulation of mitotic cell cycle spindle assembly checkpoint; ISS:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |