UniProt Accession

 MPIP2_HUMAN; P30305; D3DVY1; D3DVY2; D3DVY3; D3DVY4; O43551; Q13971; Q5JX77; Q6RSS1; Q9BRA6 

Genbank Protein ID

 M81934; S78187; X96436; Z68092; AY494082; AL109804; AL109804; AL109804; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; BC006395; BC009953; BC051711; AF036233; AF036233 

Genbank Nucleotide ID

 AAA58416.1; AAB21139.1; CAA65303.1; CAA92108.1; AAR26469.1; CAC17548.1; CAC17549.1; CAI18847.1; EAX10491.1; EAX10492.1; EAX10493.1; EAX10494.1; EAX10496.1; EAX10497.1; EAX10498.1; EAX10499.1; AAH06395.1; AAH09953.1; AAH51711.1; AAB94622.1; AAB94624.1 

Protein Name

 M-phase inducer phosphatase 2 

Protein Synonyms/Alias

 Dual specificity phosphatase Cdc25B 

Gene Name

 CDC25B 

Gene Synonyms/Alias

 CDC25HU2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
190	FVFKMPWKPTHPSST	ubiquitination	[1]
228	MCLSPDRKMEVEELS	ubiquitination	[1]
289	PLVKTLEKEEEKDLV	ubiquitination	[1]
384	ELIGDYSKAFLLQTV	ubiquitination	[1]
399	DGKHQDLKYISPETM	ubiquitination	[1]
509	YPEMYILKGGYKEFF	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. 

Sequence Annotation

 DOMAIN 431 538 Rhodanese.
 ACT_SITE 487 487
 MOD_RES 169 169 Phosphoserine; by MELK.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 323 323 Phosphoserine; by MELK and MAPK14.
 MOD_RES 353 353 Phosphoserine; by AURKA.
 MOD_RES 375 375 Phosphoserine; by BRSK1 and MAPK14.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Mitosis; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 580 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; TAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007144; P:female meiosis I; IEA:Compara.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:UniProtKB.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0001556; P:oocyte maturation; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0032467; P:positive regulation of cytokinesis; IMP:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
 GO:0045860; P:positive regulation of protein kinase activity; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IDA:UniProtKB. 

Interpro

 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 

Pfam

 PF06617; M-inducer_phosp
 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS50206; RHODANESE_3 

PRINTS

 PR00716; MPIPHPHTASE.