UniProt Accession

 BPHL_MOUSE; Q8R164; Q8BVH2; Q8R589; Q9DCC6 

Genbank Protein ID

 AK002907; AK078232; BC025162; BC023146 

Genbank Nucleotide ID

 BAB22447.1; BAC37185.1; AAH25162.1; AAH23146.1 

Protein Name

 Valacyclovir hydrolase 

Protein Synonyms/Alias

 VACVase; Valacyclovirase; Biphenyl hydrolase-like protein 

Gene Name

 Bphl 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
74	PGMLGSGKTDFAPQL	acetylation	[1, 2, 3, 4, 5, 6]
74	PGMLGSGKTDFAPQL	succinylation	[5]
86	PQLQSLNKKRFTLVA	acetylation	[3, 4, 6]
119	DFFERDAKDAVDLMK	acetylation	[2, 3, 4, 6]
126	KDAVDLMKALQFKQV	acetylation	[1, 2, 3, 4, 5, 6, 7]
126	KDAVDLMKALQFKQV	succinylation	[5]
126	KDAVDLMKALQFKQV	ubiquitination	[8]
184	QGIRDVSKWSEKARK	acetylation	[3, 5, 6]
184	QGIRDVSKWSEKARK	succinylation	[5]
188	DVSKWSEKARKPLEA	acetylation	[4, 6]
191	KWSEKARKPLEALYG	acetylation	[4, 5]
191	KWSEKARKPLEALYG	succinylation	[5]
191	KWSEKARKPLEALYG	ubiquitination	[8]
217	VDGISQFKQLPEGNI	acetylation	[4, 6]
243	TLIVHGEKDPLVPRF	acetylation	[3, 4, 6]
260	DFLLQHVKGSRLHLM	acetylation	[4, 5, 6]
260	DFLLQHVKGSRLHLM	succinylation	[5]
271	LHLMPEGKHNLHLRF	acetylation	[3, 4, 5, 6]
271	LHLMPEGKHNLHLRF	succinylation	[5]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [8] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol (By similarity). 

Sequence Annotation

 ACT_SITE 139 139 Nucleophile (By similarity).
 ACT_SITE 244 244 Charge relay system (By similarity).
 ACT_SITE 272 272 Charge relay system (By similarity).
 MOD_RES 126 126 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Reference proteome; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 291 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. 

Interpro

  

Pfam

  

SMART

  

PROSITE

 PS00120; LIPASE_SER 

PRINTS