CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030997
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sphingomyelin phosphodiesterase 4 
Protein Synonyms/Alias
 cDNA FLJ59341, highly similar to Homo sapiens sphingomyelin phosphodiesterase 4, neutral membrane, transcript variant 1, mRNA 
Gene Name
 SMPD4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
62LKADSINKPFAQQCQubiquitination[1]
202PWRYAPDKQAPGSDSubiquitination[1, 2, 3, 4, 5]
217QPRCVSEKWAPFVQEubiquitination[3, 4]
249RTDLVSPKHALMVFRubiquitination[1, 6]
317TDASFKVKSHVYSLEubiquitination[1, 3, 5]
329SLEGQDCKYTPMFGPubiquitination[1, 5]
353AQLITQAKHTAKSISubiquitination[1, 5]
402MGQDSVRKTDEYLEKubiquitination[1, 2]
409KTDEYLEKALEYLRQacetylation[7]
409KTDEYLEKALEYLRQubiquitination[1, 2, 6]
441TTQDENGKKQLPDCIubiquitination[1, 2, 5]
442TQDENGKKQLPDCIVubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 615 AA 
Protein Sequence
MTTFGAVAEW RLPSLRRATL WIPQWFAKKA IFNSPLEAAM AFPHLQQPSF LLASLKADSI 60
NKPFAQQCQD LVKVIEDFPA KVFVEMWLHH YSLEMYQKMQ SPHAKESFTP TEEHVLVVRL 120
LLKHLHAFAN SLKPEQASPS AHSHATSPLE EFKRAAVPRF VQQKLYLFLQ HCFGHWPLDA 180
SFRAVLEMWL SYLQPWRYAP DKQAPGSDSQ PRCVSEKWAP FVQENLLMYT KLFVGFLNRA 240
LRTDLVSPKH ALMVFRVAKV FAQPNLAEMI QKGEQLFLEP ELVIPHRQHR LFTAPTFTGS 300
FLSPWPPAVT DASFKVKSHV YSLEGQDCKY TPMFGPEART LVLRLAQLIT QAKHTAKSIS 360
DQCAESPAGH SFLSWLGFSS MDTNGSYTAN DLDEMGQDSV RKTDEYLEKA LEYLRQIFRL 420
SEAQLRQFTL ALGTTQDENG KKQLPDCIVG EDGLILTPLG RYQIINGLRR FEIEYQGDPE 480
LQPIRSYEIA SLVRTLFRLS SAINHRFAGQ MAALCSRDDF LGSFCRYHLT EPGLASRHLL 540
SPVGRRQVAG HTRGPRLSLR FLGSYRTLVS LLLAFFVASL FCVGPLPCTL LLTLGYVLYA 600
SAMTLLTERG KLHQP 615 
Gene Ontology
 GO:0050290; F:sphingomyelin phosphodiesterase D activity; IEA:InterPro. 
Interpro
 IPR024129; Sphingomy_SMPD4. 
Pfam
  
SMART
  
PROSITE
  
PRINTS