CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015604
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carnitine O-acetyltransferase 
Protein Synonyms/Alias
 Carnitine acetylase; Carnitine acetyltransferase; CAT; CrAT 
Gene Name
 Crat 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
52QSLDHYLKALQPIVSacetylation[1]
93GLERRAKKMENWLSEacetylation[1]
186SCRVPGLKQDSVVNFacetylation[1]
195DSVVNFLKSKKPPTHacetylation[1]
245NSSLQSNKEPVGILTacetylation[1]
261NHRNSWAKAYSSLIKacetylation[1]
268KAYSSLIKDKVNRESacetylation[1]
315MLHGGGSKFNSGNRWacetylation[1]
390FNITPEIKNDIEKAKacetylation[1]
395EIKNDIEKAKQNISIacetylation[1]
480TDSLAFVKGMDDPKVacetylation[1]
486VKGMDDPKVPEQQRVacetylation[1]
609RMAHYLEKALLDMRTacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria (By similarity). 
Sequence Annotation
 REGION 418 430 Coenzyme A binding (By similarity).
 MOTIF 624 626 Microbody targeting signal (Potential).
 ACT_SITE 343 343 Proton acceptor.
 BINDING 452 452 Carnitine (By similarity).
 BINDING 454 454 Carnitine (By similarity).
 BINDING 455 455 Coenzyme A (By similarity).
 BINDING 465 465 Carnitine (By similarity).
 MOD_RES 261 261 N6-acetyllysine (By similarity).
 MOD_RES 268 268 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Acyltransferase; Complete proteome; Endoplasmic reticulum; Fatty acid metabolism; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion inner membrane; Peroxisome; Reference proteome; Transferase; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 626 AA 
Protein Sequence
MLAFAARTVV KPLGLLKPSS LMKVSGRFKA HQDALPRLPV PPLQQSLDHY LKALQPIVSE 60
EEWAHTKQLV DEFQTSGGVG ERLQKGLERR AKKMENWLSE WWLKTAYLQF RQPVVIYSSP 120
GVLLPKQDFM DLQGQLRFAA KLIEGVLDFK SMIDNETLPV EFLGGQPLCM NQYYQILSSC 180
RVPGLKQDSV VNFLKSKKPP THITVVHNYQ FFELDVYHSD GTPLTSDQIF VQLEKIWNSS 240
LQSNKEPVGI LTSNHRNSWA KAYSSLIKDK VNRESVNSIQ KSIFTVCLDK QVPRVSDDVY 300
RSHVAGQMLH GGGSKFNSGN RWFDKTLQFI VAEDGSCGMV YEHAAAEGPP IVALVDHVME 360
YTKKPELVRS PMVPLPMPKK LRFNITPEIK NDIEKAKQNI SIMIQDLDIM MLVFHHFGKD 420
FPKSQKLSPD AFIQIALQLA YYRIYGQACA TYESASLRMF HLGRTDTIRS ASTDSLAFVK 480
GMDDPKVPEQ QRVELLRKAV QAHRAYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD 540
TSYAIAMHFN LSTSQVPAKT DCVMSFGPVV PDGYGICYNP MEAHINFSVS AYNSCAETNA 600
ARMAHYLEKA LLDMRTLLQN HPRAKL 626 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:RGD.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; TAS:RGD.
 GO:0005777; C:peroxisome; TAS:RGD.
 GO:0004092; F:carnitine O-acetyltransferase activity; IDA:RGD.
 GO:0006631; P:fatty acid metabolic process; IDA:RGD.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR000542; Carn_acyl_trans. 
Pfam
 PF00755; Carn_acyltransf 
SMART
  
PROSITE
 PS00439; ACYLTRANSF_C_1
 PS00440; ACYLTRANSF_C_2 
PRINTS