UniProt Accession

 PURT_ECOLI; P33221 

Genbank Protein ID

 L20897; U00096; AP009048 

Genbank Nucleotide ID

 AAA23861.1; AAC74919.1; BAA15657.1 

Protein Name

 Phosphoribosylglycinamide formyltransferase 2 

Protein Synonyms/Alias

 GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 

Gene Name

 purT 

Gene Synonyms/Alias

 b1849; JW1838 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
109	VPCARATKLTMNREG	acetylation	[1]
179	EQLAQAWKYAQQGGR	acetylation	[1, 2]
355	LQIRLFGKPEIDGSR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP. 

Sequence Annotation

 DOMAIN 119 308 ATP-grasp.
 NP_BIND 160 165 ATP.
 NP_BIND 195 198 ATP.
 REGION 22 23 5'-phosphoribosylglycinamide binding.
 REGION 362 363 5'-phosphoribosylglycinamide binding.
 METAL 267 267 Magnesium (By similarity).
 METAL 279 279 Magnesium (By similarity).
 BINDING 82 82 5'-phosphoribosylglycinamide.
 BINDING 114 114 ATP.
 BINDING 155 155 ATP.
 BINDING 203 203 ATP.
 BINDING 267 267 ATP.
 BINDING 279 279 ATP.
 BINDING 286 286 5'-phosphoribosylglycinamide.
 BINDING 355 355 5'-phosphoribosylglycinamide.
 MOD_RES 179 179 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 392 AA 

Protein Sequence

Gene Ontology

 GO:0008776; F:acetate kinase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0043815; F:phosphoribosylglycinamide formyltransferase 2 activity; IEA:HAMAP.
 GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IEA:InterPro.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR011761; ATP-grasp.
 IPR003135; ATP-grasp_carboxylate-amine.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR005862; PurT.
 IPR011054; Rudment_hybrid_motif. 

Pfam

 PF02222; ATP-grasp 

SMART

  

PROSITE

 PS50975; ATP_GRASP 

PRINTS