CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012634
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear receptor coactivator 1 
Protein Synonyms/Alias
 NCoA-1; Class E basic helix-loop-helix protein 74; bHLHe74; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; Steroid receptor coactivator 1; SRC-1 
Gene Name
 NCOA1 
Gene Synonyms/Alias
 BHLHE74; SRC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
732VQGNSSIKLELDASKsumoylation[1]
774SLDDVKVKVEKKEQMsumoylation[1]
Reference
 [1] Sumoylation of the progesterone receptor and of the steroid receptor coactivator SRC-1.
 Chauchereau A, Amazit L, Quesne M, Guiochon-Mantel A, Milgrom E.
 J Biol Chem. 2003 Apr 4;278(14):12335-43. [PMID: 12529333] 
Functional Description
 Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. 
Sequence Annotation
 DOMAIN 23 80 bHLH.
 DOMAIN 109 180 PAS.
 REGION 361 567 Interaction with STAT3.
 REGION 781 988 Interaction with CREBBP.
 MOTIF 46 50 LXXLL motif 1.
 MOTIF 112 116 LXXLL motif 2.
 MOTIF 633 637 LXXLL motif 3.
 MOTIF 690 694 LXXLL motif 4.
 MOTIF 749 753 LXXLL motif 5.
 MOTIF 913 917 LXXLL motif 6.
 MOTIF 1435 1439 LXXLL motif 7.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 22 22 Phosphoserine (By similarity).
 MOD_RES 372 372 Phosphoserine.
 MOD_RES 395 395 Phosphoserine.
 MOD_RES 517 517 Phosphoserine.
 MOD_RES 569 569 Phosphoserine.
 MOD_RES 1033 1033 Phosphoserine.
 MOD_RES 1179 1179 Phosphothreonine.
 MOD_RES 1185 1185 Phosphoserine.
 CROSSLNK 732 732 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 774 774 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Activator; Acyltransferase; Alternative splicing; Chromosomal rearrangement; Complete proteome; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; Repeat; Transcription; Transcription regulation; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1441 AA 
Protein Sequence
MSGLGDSSSD PANPDSHKRK GSPCDTLASS TEKRRREQEN KYLEELAELL SANISDIDSL 60
SVKPDKCKIL KKTVDQIQLM KRMEQEKSTT DDDVQKSDIS SSSQGVIEKE SLGPLLLEAL 120
DGFFFVVNCE GRIVFVSENV TSYLGYNQEE LMNTSVYSIL HVGDHAEFVK NLLPKSLVNG 180
VPWPQEATRR NSHTFNCRML IHPPDEPGTE NQEACQRYEV MQCFTVSQPK SIQEDGEDFQ 240
SCLICIARRL PRPPAITGVE SFMTKQDTTG KIISIDTSSL RAAGRTGWED LVRKCIYAFF 300
QPQGREPSYA RQLFQEVMTR GTASSPSYRF ILNDGTMLSA HTKCKLCYPQ SPDMQPFIMG 360
IHIIDREHSG LSPQDDTNSG MSIPRVNPSV NPSISPAHGV ARSSTLPPSN SNMVSTRINR 420
QQSSDLHSSS HSNSSNSQGS FGCSPGSQIV ANVALNQGQA SSQSSNPSLN LNNSPMEGTG 480
ISLAQFMSPR RQVTSGLATR PRMPNNSFPP NISTLSSPVG MTSSACNNNN RSYSNIPVTS 540
LQGMNEGPNN SVGFSASSPV LRQMSSQNSP SRLNIQPAKA ESKDNKEIAS ILNEMIQSDN 600
SSSDGKPLDS GLLHNNDRLS DGDSKYSQTS HKLVQLLTTT AEQQLRHADI DTSCKDVLSC 660
TGTSNSASAN SSGGSCPSSH SSLTERHKIL HRLLQEGSPS DITTLSVEPD KKDSASTSVS 720
VTGQVQGNSS IKLELDASKK KESKDHQLLR YLLDKDEKDL RSTPNLSLDD VKVKVEKKEQ 780
MDPCNTNPTP MTKPTPEEIK LEAQSQFTAD LDQFDQLLPT LEKAAQLPGL CETDRMDGAV 840
TSVTIKSEIL PASLQSATAR PTSRLNRLPE LELEAIDNQF GQPGTGDQIP WTNNTVTAIN 900
QSKSEDQCIS SQLDELLCPP TTVEGRNDEK ALLEQLVSFL SGKDETELAE LDRALGIDKL 960
VQGGGLDVLS ERFPPQQATP PLIMEERPNL YSQPYSSPSP TANLPSPFQG MVRQKPSLGT 1020
MPVQVTPPRG AFSPGMGMQP RQTLNRPPAA PNQLRLQLQQ RLQGQQQLIH QNRQAILNQF 1080
AATAPVGINM RSGMQQQITP QPPLNAQMLA QRQRELYSQQ HRQRQLIQQQ RAMLMRQQSF 1140
GNNLPPSSGL PVQMGNPRLP QGAPQQFPYP PNYGTNPGTP PASTSPFSQL AANPEASLAN 1200
RNSMVSRGMT GNIGGQFGTG INPQMQQNVF QYPGAGMVPQ GEANFAPSLS PGSSMVPMPI 1260
PPPQSSLLQQ TPPASGYQSP DMKAWQQGAI GNNNVFSQAV QNQPTPAQPG VYNNMSITVS 1320
MAGGNTNVQN MNPMMAQMQM SSLQMPGMNT VCPEQINDPA LRHTGLYCNQ LSSTDLLKTE 1380
ADGTQQVQQV QVFADVQCTV NLVGGDPYLN QPGPLGTQKP TSGPQTPQAQ QKSLLQQLLT 1440
E 1441 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IDA:UniProtKB.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; NAS:BHF-UCL.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0043967; P:histone H4 acetylation; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0045666; P:positive regulation of neuron differentiation; IEA:Compara.
 GO:0000435; P:positive regulation of transcription from RNA polymerase II promoter by galactose; IDA:UniProtKB.
 GO:2001038; P:regulation of cellular response to drug; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR011598; bHLH_dom.
 IPR010011; DUF1518.
 IPR009110; Nuc_rcpt_coact.
 IPR014920; Nuc_rcpt_coact_Ncoa-typ.
 IPR017426; Nuclear_rcpt_coactivator.
 IPR000014; PAS.
 IPR013767; PAS_fold.
 IPR014935; SRC-1.
 IPR008955; Src1_rcpt_coact. 
Pfam
 PF07469; DUF1518
 PF08815; Nuc_rec_co-act
 PF00989; PAS
 PF08832; SRC-1 
SMART
 SM00353; HLH
 SM00091; PAS 
PROSITE
 PS50888; BHLH
 PS50112; PAS 
PRINTS