UniProt Accession

 ASSY_MOUSE; P16460 

Genbank Protein ID

 M31690; M31692; M31694; M31693; M31695; M31702; BC002074; BC087556 

Genbank Nucleotide ID

 AAA37266.1; AAB60707.1; AAB60708.1; AAB60708.1; AAB60708.1; AAB60706.1; AAH02074.1; AAH87556.1 

Protein Name

 Argininosuccinate synthase 

Protein Synonyms/Alias

 Citrulline--aspartate ligase 

Gene Name

 Ass1 

Gene Synonyms/Alias

 Ass 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
41	YLANIGQKEDFEEAR	ubiquitination	[1]
53	EARKKALKLGAKKVF	acetylation	[2]
58	ALKLGAKKVFIEDVS	ubiquitination	[1]
112	IAQREGAKYVSHGAT	acetylation	[2]
112	IAQREGAKYVSHGAT	ubiquitination	[1]
121	VSHGATGKGNDQVRF	acetylation	[2]
121	VSHGATGKGNDQVRF	ubiquitination	[1]
140	YSLAPQIKVIAPWRM	ubiquitination	[1]
155	PEFYNRFKGRNDLME	ubiquitination	[1]
165	NDLMEYAKQHGIPIP	acetylation	[3]
165	NDLMEYAKQHGIPIP	ubiquitination	[1]
176	IPIPVTPKSPWSMDE	ubiquitination	[1]
199	AGILENPKNQAPPGL	ubiquitination	[1]
209	APPGLYTKTQDPAKA	ubiquitination	[1]
215	TKTQDPAKAPNSPDV	acetylation	[3]
215	TKTQDPAKAPNSPDV	ubiquitination	[1]
228	DVLEIEFKKGVPVKV	acetylation	[2]
228	DVLEIEFKKGVPVKV	ubiquitination	[1]
234	FKKGVPVKVTNIKDG	ubiquitination	[1]
239	PVKVTNIKDGTTRTT	acetylation	[2]
239	PVKVTNIKDGTTRTT	ubiquitination	[1]
260	YLNEVAGKHGVGRID	acetylation	[2]
260	YLNEVAGKHGVGRID	ubiquitination	[1]
277	ENRFIGMKSRGIYET	ubiquitination	[1]
310	DREVRKIKQGLGLKF	ubiquitination	[1]
340	FVRHCIQKSQERVEG	acetylation	[2]
340	FVRHCIQKSQERVEG	ubiquitination	[1]
408	EYHRLQSKVTAK***	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Is indirectly involved in the control of blood pressure (By similarity). 

Sequence Annotation

 NP_BIND 10 18 ATP (By similarity).
 NP_BIND 115 123 ATP (By similarity).
 BINDING 36 36 ATP; via amide nitrogen and carbonyl
 BINDING 87 87 Citrulline (By similarity).
 BINDING 92 92 Citrulline (By similarity).
 BINDING 119 119 Aspartate (By similarity).
 BINDING 123 123 Aspartate (By similarity).
 BINDING 123 123 Citrulline (By similarity).
 BINDING 124 124 Aspartate (By similarity).
 BINDING 127 127 Citrulline (By similarity).
 BINDING 180 180 Citrulline (By similarity).
 BINDING 189 189 Citrulline (By similarity).
 BINDING 270 270 Citrulline (By similarity).
 BINDING 282 282 Citrulline (By similarity).
 MOD_RES 180 180 Phosphoserine (By similarity).  

Keyword

 Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Urea cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 412 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004055; F:argininosuccinate synthase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0000050; P:urea cycle; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001518; Arginosuc_synth.
 IPR018223; Arginosuc_synth_CS.
 IPR023434; Arginosuc_synth_type_1_subfam.
 IPR024074; AS_cat/multimer_dom_body.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00764; Arginosuc_synth 

SMART

  

PROSITE

 PS00564; ARGININOSUCCIN_SYN_1
 PS00565; ARGININOSUCCIN_SYN_2 

PRINTS