CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007061
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein SMUBP-2 
Protein Synonyms/Alias
 ATP-dependent helicase IGHMBP2; Glial factor 1; GF-1; Immunoglobulin mu-binding protein 2 
Gene Name
 IGHMBP2 
Gene Synonyms/Alias
 SMBP2; SMUBP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
259VERLALCKQRILRLGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 5' to 3' helicase that unwinds RNA and DNA duplices in an ATP-dependent reaction. Acts as a transcription regulator. Required for the transcriptional activation of the flounder liver- type antifreeze protein gene. Exhibits strong binding specificity to the enhancer element B of the flounder antifreeze protein gene intron. Binds to the insulin II gene RIPE3B enhancer region. May be involved in translation (By similarity). DNA-binding protein specific to 5'-phosphorylated single-stranded guanine-rich sequence related to the immunoglobulin mu chain switch region. Preferentially binds to the 5'-GGGCT-3' motif. Interacts with tRNA-Tyr. Stimulates the transcription of the human neurotropic virus JCV. 
Sequence Annotation
 DOMAIN 723 786 R3H.
 NP_BIND 214 221 ATP (By similarity).
 ZN_FING 894 940 AN1-type.
 REGION 638 785 SS DNA-binding (By similarity).
 MOTIF 864 868 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Activator; ATP-binding; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; DNA-binding; Helicase; Hydrolase; Metal-binding; Neurodegeneration; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation; tRNA-binding; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 993 AA 
Protein Sequence
MASAAVESFV TKQLDLLELE RDAEVEERRS WQENISLKEL QSRGVCLLKL QVSSQRTGLY 60
GRLLVTFEPR RYGSAAALPS NSFTSGDIVG LYDAANEGSQ LATGILTRVT QKSVTVAFDE 120
SHDFQLSLDR ENSYRLLKLA NDVTYRRLKK ALIALKKYHS GPASSLIEVL FGRSAPSPAS 180
EIHPLTFFNT CLDTSQKEAV LFALSQKELA IIHGPPGTGK TTTVVEIILQ AVKQGLKVLC 240
CAPSNIAVDN LVERLALCKQ RILRLGHPAR LLESIQQHSL DAVLARSDSA QIVADIRKDI 300
DQVFVKNKKT QDKREKSNFR NEIKLLRKEL KEREEAAMLE SLTSANVVLA TNTGASADGP 360
LKLLPESYFD VVVIDECAQA LEASCWIPLL KARKCILAGD HKQLPPTTVS HKAALAGLSL 420
SLMERLAEEY GARVVRTLTV QYRMHQAIMR WASDTMYLGQ LTAHSSVARH LLRDLPGVAA 480
TEETGVPLLL VDTAGCGLFE LEEEDEQSKG NPGEVRLVSL HIQALVDAGV PARDIAVVSP 540
YNLQVDLLRQ SLVHRHPELE IKSVDGFQGR EKEAVILSFV RSNRKGEVGF LAEDRRINVA 600
VTRARRHVAV ICDSRTVNNH AFLKTLVEYF TQHGEVRTAF EYLDDIVPEN YSHENSQGSS 660
HAATKPQGPA TSTRTGSQRQ EGGQEAAAPA RQGRKKPAGK SLASEAPSQP SLNGGSPEGV 720
ESQDGVDHFR AMIVEFMASK KMQLEFPPSL NSHDRLRVHQ IAEEHGLRHD SSGEGKRRFI 780
TVSKRAPRPR AALGPPAGTG GPAPLQPVPP TPAQTEQPPR EQRGPDQPDL RTLHLERLQR 840
VRSAQGQPAS KEQQASGQQK LPEKKKKKAK GHPATDLPTE EDFEALVSAA VKADNTCGFA 900
KCTAGVTTLG QFCQLCSRRY CLSHHLPEIH GCGERARAHA RQRISREGVL YAGSGTKNGS 960
LDPAKRAQLQ RRLDKKLSEL SNQRTSRRKE RGT 993 
Gene Ontology
 GO:0030424; C:axon; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030426; C:growth cone; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0043141; F:ATP-dependent 5'-3' DNA helicase activity; IDA:UniProtKB.
 GO:0032575; F:ATP-dependent 5'-3' RNA helicase activity; IDA:UniProtKB.
 GO:0043022; F:ribosome binding; IDA:UniProtKB.
 GO:0003697; F:single-stranded DNA binding; TAS:ProtInc.
 GO:0000049; F:tRNA binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0006310; P:DNA recombination; TAS:ProtInc.
 GO:0006281; P:DNA repair; TAS:ProtInc.
 GO:0006260; P:DNA replication; TAS:ProtInc.
 GO:0051260; P:protein homooligomerization; IPI:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006412; P:translation; NAS:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR004483; DNA_helicase_put.
 IPR014001; Helicase_ATP-bd.
 IPR027417; P-loop_NTPase.
 IPR001374; R3H_ss-bd.
 IPR026853; SMUBP-2.
 IPR000058; Znf_AN1. 
Pfam
 PF01424; R3H
 PF01428; zf-AN1 
SMART
 SM00382; AAA
 SM00487; DEXDc
 SM00393; R3H
 SM00154; ZnF_AN1 
PROSITE
 PS51061; R3H
 PS51039; ZF_AN1 
PRINTS