CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029142
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polypyrimidine tract binding protein 1, isoform CRA_e 
Protein Synonyms/Alias
 Polypyrimidine tract-binding protein 1 
Gene Name
 PTBP1 
Gene Synonyms/Alias
 hCG_20560 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13PDIAVGTKRVTPQSLubiquitination[1, 2, 3, 4]
34YGDVQRVKILFNKKEacetylation[4]
34YGDVQRVKILFNKKEubiquitination[5]
76PIRITLSKHQNVQLPacetylation[4, 6]
76PIRITLSKHQNVQLPubiquitination[3, 4, 5, 7]
94QEDQGLTKDYGNSPLubiquitination[3, 4, 5]
148SSNGGVVKGFKFFQKubiquitination[2, 3, 4, 5, 7, 8]
151GGVVKGFKFFQKDRKacetylation[4]
151GGVVKGFKFFQKDRKubiquitination[3, 4, 5]
155KGFKFFQKDRKMALIubiquitination[3]
194HLRVSFSKSTI****ubiquitination[2, 3, 7, 8]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 197 AA 
Protein Sequence
MDGIVPDIAV GTKRVTPQSL FILFGVYGDV QRVKILFNKK ENALVQMADG NQAQLAMSHL 60
NGHKLHGKPI RITLSKHQNV QLPREGQEDQ GLTKDYGNSP LHRFKKPGSK NFQNIFPPSA 120
TLHLSNIPPS VSEEDLKVLF SSNGGVVKGF KFFQKDRKMA LIQMGSVEEA VQALIDLHNH 180
DLGENHHLRV SFSKSTI 197 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:InterPro. 
Interpro
 IPR006536; HnRNP-L_PTB.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
  
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS