CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008152
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein phosphatase 1A 
Protein Synonyms/Alias
 Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA 
Gene Name
 Ppm1a 
Gene Synonyms/Alias
 Pppm1a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
98APSVENVKNGIRTGFubiquitination[1]
322RVEEIIKKQVEGVPDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). 
Sequence Annotation
 METAL 60 60 Manganese 1 (By similarity).
 METAL 60 60 Manganese 2 (By similarity).
 METAL 61 61 Manganese 1; via carbonyl oxygen (By
 METAL 239 239 Manganese 2 (By similarity).
 METAL 282 282 Manganese 2 (By similarity).
 MOD_RES 375 375 Phosphoserine (By similarity).
 MOD_RES 377 377 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine (By similarity).  
Keyword
 Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 382 AA 
Protein Sequence
MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 60
GHAGSQVAKY CCEHLLDHIT NNQDFRGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 120
GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 180
SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 240
IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPSAPKVSAE 300
AVKKEAELDK YLESRVEEII KKQVEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 360
VYNRLNPYKN DDTDSASTDD MW 382 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005891; C:voltage-gated calcium channel complex; IEA:Compara.
 GO:0033192; F:calmodulin-dependent protein phosphatase activity; IEA:Compara.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0004721; F:phosphoprotein phosphatase activity; IDA:MGI.
 GO:0070412; F:R-SMAD binding; ISS:UniProtKB.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0006499; P:N-terminal protein myristoylation; IDA:UniProtKB.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB.
 GO:0042347; P:negative regulation of NF-kappaB import into nucleus; ISS:UniProtKB.
 GO:0010991; P:negative regulation of SMAD protein complex assembly; IEA:Compara.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IEA:Compara.
 GO:0035970; P:peptidyl-threonine dephosphorylation; IEA:Compara.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; IMP:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:Compara. 
Interpro
 IPR001932; PP2C-like.
 IPR012911; PP2C_C.
 IPR000222; PP2C_Mn2_Asp60_BS.
 IPR015655; Protein_Pase_2C. 
Pfam
 PF00481; PP2C
 PF07830; PP2C_C 
SMART
 SM00331; PP2C_SIG
 SM00332; PP2Cc 
PROSITE
 PS01032; PP2C 
PRINTS