CPLM-024000

Genbank Nucleotide ID

Protein Synonyms/Alias

EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1

Homo sapiens (Human)

Position	Peptide	Type	References
11	SSLRRQMKNIVHNYS	ubiquitination	[1, 2, 3, 4, 5, 6]
23	NYSEAEIKVREATSN	ubiquitination	[1]
86	TLMEYLIKTGSERVS	ubiquitination	[1, 2, 4, 5]
97	ERVSQQCKENMYAVQ	ubiquitination	[1, 3, 4, 6]
107	MYAVQTLKDFQYVDR	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11]
117	QYVDRDGKDQGVNVR	ubiquitination	[1, 2, 3, 4, 5, 9]
128	VNVREKAKQLVALLR	ubiquitination	[1, 3, 4]
149	EERAHALKTKEKLAQ	ubiquitination	[2]
246	QMAIEESKRETGGKE	ubiquitination	[2, 4, 5]
469	TPTPPTRKTPESFLG	ubiquitination	[1]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
[8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[9] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[11] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.

DOMAIN 12 144 ENTH.
REPEAT 183 202 UIM 1.
REPEAT 208 227 UIM 2.
REPEAT 233 252 UIM 3.
REPEAT 274 276 1.
REPEAT 294 296 2.
REPEAT 306 308 3.
REPEAT 319 321 4.
REPEAT 332 334 5.
REPEAT 349 351 6.
REPEAT 367 369 7.
REPEAT 377 379 8.
REPEAT 502 504 1.
REPEAT 518 520 2.
REPEAT 572 574 3.
REGION 274 379 8 X 3 AA repeats of [ED]-P-W.
REGION 502 574 3 X 3 AA repeats of N-P-F.
MOTIF 402 411 [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif.
BINDING 8 8 Phosphatidylinositol lipid headgroup (By
BINDING 11 11 Phosphatidylinositol lipid headgroup (By
BINDING 25 25 Phosphatidylinositol lipid headgroup (By
BINDING 30 30 Phosphatidylinositol lipid headgroup (By
BINDING 63 63 Phosphatidylinositol lipid headgroup (By
BINDING 73 73 Phosphatidylinositol lipid headgroup (By
MOD_RES 382 382 Phosphoserine; by CDK1.
MOD_RES 419 419 Phosphoserine.
MOD_RES 420 420 Phosphoserine.
MOD_RES 435 435 Phosphoserine.
MOD_RES 454 454 Phosphoserine.
MOD_RES 460 460 Phosphothreonine.
MOD_RES 470 470 Phosphothreonine.
MOD_RES 494 494 Phosphothreonine.

3D-structure; Alternative splicing; Cell membrane; Coated pit; Complete proteome; Cytoplasm; Endocytosis; Lipid-binding; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
GO:0048568; P:embryonic organ development; IEA:Compara.
GO:0006897; P:endocytosis; TAS:ProtInc.
GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0007565; P:female pregnancy; IEA:Compara.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0007219; P:Notch signaling pathway; IEA:Compara.

IPR008942; ENTH_VHS.
IPR027320; Epsin-1_mammalian.
IPR013809; Epsin-like_N.
IPR001026; Epsin_dom_N.
IPR003903; Ubiquitin-int_motif.

PF01417; ENTH
PF02809; UIM

SM00273; ENTH
SM00726; UIM

PS50942; ENTH
PS50330; UIM