UniProt Accession

 RPAB1_YEAST; P20434; D6VQE9; Q02121 

Genbank Protein ID

 X53287; X71329; S59774; Z36023; BK006936 

Genbank Nucleotide ID

 CAA37381.1; CAA50472.1; AAC60556.1; CAA85113.1; DAA07269.1 

Protein Name

 DNA-directed RNA polymerases I, II, and III subunit RPABC1 

Protein Synonyms/Alias

 RNA polymerases I, II, and III subunit ABC1; ABC27; DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide 

Gene Name

 RPB5 

Gene Synonyms/Alias

 RPA7; RPC9; YBR154C; YBR1204 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
171	LLKRYRLKESQLPRI	ubiquitination	[1]
191	VALYLGLKRGEVVKI	ubiquitination	[1]
197	LKRGEVVKIIRKSET	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity). 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; DNA-binding; DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0005736; C:DNA-directed RNA polymerase I complex; IDA:SGD.
 GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:SGD.
 GO:0005666; C:DNA-directed RNA polymerase III complex; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0006360; P:transcription from RNA polymerase I promoter; IDA:SGD.
 GO:0006366; P:transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IDA:SGD. 

Interpro

 IPR014381; DNA_RNA_pol_RPB5_euk/virus.
 IPR005571; RNA_pol_Rpb5_N.
 IPR000783; RNA_pol_subH/Rpb5_C.
 IPR020608; RNA_pol_subH/Rpb5_CS.
 IPR020609; RpoH/RPB5. 

Pfam

 PF01191; RNA_pol_Rpb5_C
 PF03871; RNA_pol_Rpb5_N 

SMART

  

PROSITE

 PS01110; RNA_POL_H_23KD 

PRINTS