CPLM-030751

Genbank Nucleotide ID

Isocitrate dehydrogenase [NADP]

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
15	RIIWQFIKEKLILPH	ubiquitination	[1]
17	IWQFIKEKLILPHVD	acetylation	[2]
28	PHVDIQLKYFDLGLP	acetylation	[2]
54	DSALATQKYSVAVKC	acetylation	[2, 3]
60	QKYSVAVKCATITPD	ubiquitination	[1]
75	EARVEEFKLKKMWKS	acetylation	[2]
75	EARVEEFKLKKMWKS	ubiquitination	[1, 2]
77	RVEEFKLKKMWKSPN	ubiquitination	[1]
103	FREPIICKNIPRLVP	acetylation	[2]
114	RLVPGWTKPITIGRH	acetylation	[2]
114	RLVPGWTKPITIGRH	ubiquitination	[1, 2, 4, 5]
128	HAHGDQYKATDFVAD	acetylation	[2, 3, 6]
128	HAHGDQYKATDFVAD	ubiquitination	[1, 5]
141	ADRAGTFKMVFTPKD	ubiquitination	[1]
204	STKNTILKAYDGRFK	ubiquitination	[2]
211	KAYDGRFKDIFQEIF	acetylation	[2, 6]
211	KAYDGRFKDIFQEIF	ubiquitination	[4]
220	IFQEIFDKHYKTDFD	acetylation	[2]
220	IFQEIFDKHYKTDFD	ubiquitination	[1, 4]
228	HYKTDFDKNKIWYEH	acetylation	[2]
228	HYKTDFDKNKIWYEH	ubiquitination	[5]
230	KTDFDKNKIWYEHRL	acetylation	[2]
332	RGLEHRGKLDGNQDL	acetylation	[2, 3]
332	RGLEHRGKLDGNQDL	ubiquitination	[1]
361	VESGAMTKDLAGCIH	acetylation	[2]
361	VESGAMTKDLAGCIH	ubiquitination	[1]
390	TDFLDTIKSNLDRAL	acetylation	[3]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]

Functional Description

NP_BIND 63 65 NADP (By similarity).
NP_BIND 297 302 NADP (By similarity).
REGION 82 88 Substrate binding (By similarity).
METAL 239 239 Magnesium or manganese (By similarity).
METAL 262 262 Magnesium or manganese (By similarity).
BINDING 65 65 Substrate (By similarity).
BINDING 70 70 NADP (By similarity).
BINDING 97 97 Substrate (By similarity).
BINDING 120 120 Substrate (By similarity).
BINDING 247 247 NADP (By similarity).
BINDING 315 315 NADP; via amide nitrogen and carbonyl

Complete proteome; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
GO:0005739; C:mitochondrion; IDA:HPA.
GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:EC.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0051287; F:NAD binding; IEA:InterPro.
GO:0006102; P:isocitrate metabolic process; IEA:InterPro.
GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.

IPR019818; IsoCit/isopropylmalate_DH_CS.
IPR004790; Isocitrate_DH_NADP.
IPR024084; IsoPropMal-DH-like_dom.