CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018996
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nicalin 
Protein Synonyms/Alias
 Nicastrin-like protein 
Gene Name
 NCLN 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99FSYEQYQKALRQSAGubiquitination[1, 2, 3, 4, 5, 6]
194VTSGVQSKAVSDWLIubiquitination[2]
262FSRLYTYKRTHAAYNubiquitination[5]
286KFNYQGTKRWLEDNLubiquitination[2, 5]
326SLHLHVSKPPREGTLubiquitination[2, 5]
359VRFSMVHKRINLAEDubiquitination[5]
409VRSRVDSKTLTRNTRubiquitination[5]
471RAAQLVDKDSTFLSTubiquitination[2, 5]
488HHLSRYLKDVKQHHVubiquitination[5]
491SRYLKDVKQHHVKADubiquitination[2, 5]
499QHHVKADKRDPEFVFubiquitination[2, 5]
511FVFYDQLKQVMNAYRubiquitination[2]
559TVQRLLVKAKTQ***ubiquitination[2]
561QRLLVKAKTQ*****ubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning (By similarity). 
Sequence Annotation
 CARBOHYD 241 241 N-linked (GlcNAc...) (Potential).
 CARBOHYD 428 428 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Glycoprotein; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 563 AA 
Protein Sequence
MLEEAGEVLE NMLKASCLPL GFIVFLPAVL LLVAPPLPAA DAAHEFTVYR MQQYDLQGQP 60
YGTRNAVLNT EARTMAAEVL SRRCVLMRLL DFSYEQYQKA LRQSAGAVVI ILPRAMAAVP 120
QDVVRQFMEI EPEMLAMETA VPVYFAVEDE ALLSIYKQTQ AASASQGSAS AAEVLLRTAT 180
ANGFQMVTSG VQSKAVSDWL IASVEGRLTG LGGEDLPTIV IVAHYDAFGV APWLSLGADS 240
NGSGVSVLLE LARLFSRLYT YKRTHAAYNL LFFASGGGKF NYQGTKRWLE DNLDHTDSSL 300
LQDNVAFVLC LDTVGRGSSL HLHVSKPPRE GTLQHAFLRE LETVAAHQFP EVRFSMVHKR 360
INLAEDVLAW EHERFAIRRL PAFTLSHLES HRDGQRSSIM DVRSRVDSKT LTRNTRIIAE 420
ALTRVIYNLT EKGTPPDMPV FTEQMQIQQE QLDSVMDWLT NQPRAAQLVD KDSTFLSTLE 480
HHLSRYLKDV KQHHVKADKR DPEFVFYDQL KQVMNAYRVK PAVFDLLLAV GIAAYLGMAY 540
VAVQHFSLLY KTVQRLLVKA KTQ 563 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008233; F:peptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:InterPro.
 GO:0009966; P:regulation of signal transduction; IEA:InterPro. 
Interpro
 IPR016574; Nicalin.
 IPR007484; Peptidase_M28. 
Pfam
 PF04389; Peptidase_M28 
SMART
  
PROSITE
  
PRINTS