UniProt Accession

 TRY3_HUMAN; P35030; A8CED1; A9Z1Y4; E7ES07; P15951; Q15665; Q5VXV0; Q9UQV3 

Genbank Protein ID

 X72781; X71345; X15505; D45417; AB298285; AL356489; AL139113; AL358573; AL358573; AL139113; AL358573; AL139113; AL356489; AL139113; AL358573; AL139113; AL356489; AL358573; CH471071 

Genbank Nucleotide ID

 CAB58178.1; CAA50484.1; CAA33527.1; BAA08257.1; BAF80324.1; CAH69873.1; CAH69873.1; CAH69873.1; CAI39514.1; CAI39514.1; CAI39515.1; CAI39515.1; CAI39515.1; CAI39655.1; CAI39655.1; CAI39658.1; CAI39658.1; CAI39658.1; EAW58484.1 

Protein Name

 Trypsin-3 

Protein Synonyms/Alias

 Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV 

Gene Name

 PRSS3 

Gene Synonyms/Alias

 PRSS4; TRY3; TRY4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
149	EQFINAAKIIRHPKY	ubiquitination	[1]
169	DNDIMLIKLSSPAVI	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5. 

Sequence Annotation

 DOMAIN 81 301 Peptidase S1.
 ACT_SITE 120 120 Charge relay system.
 ACT_SITE 164 164 Charge relay system.
 ACT_SITE 257 257 Charge relay system.
 METAL 132 132 Calcium.
 METAL 134 134 Calcium; via carbonyl oxygen.
 METAL 137 137 Calcium; via carbonyl oxygen.
 METAL 142 142 Calcium.
 MOD_RES 211 211 Sulfotyrosine (By similarity).
 DISULFID 87 217
 DISULFID 105 121
 DISULFID 196 263
 DISULFID 228 242
 DISULFID 253 277  

Keyword

 3D-structure; Alternative splicing; Calcium; Complete proteome; Digestion; Disulfide bond; Hydrolase; Metal-binding; Polymorphism; Protease; Reference proteome; Secreted; Serine protease; Signal; Sulfation; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 304 AA 

Protein Sequence

Gene Ontology

 GO:0005615; C:extracellular space; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0004252; F:serine-type endopeptidase activity; IDA:UniProtKB.
 GO:0008236; F:serine-type peptidase activity; IDA:UniProtKB.
 GO:0007586; P:digestion; TAS:UniProtKB.
 GO:0043542; P:endothelial cell migration; IMP:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006508; P:proteolysis; IDA:UniProtKB.
 GO:0031638; P:zymogen activation; IDA:UniProtKB. 

Interpro

 IPR001254; Peptidase_S1.
 IPR018114; Peptidase_S1_AS.
 IPR001314; Peptidase_S1A.
 IPR009003; Trypsin-like_Pept_dom. 

Pfam

 PF00089; Trypsin 

SMART

 SM00020; Tryp_SPc 

PROSITE

 PS50240; TRYPSIN_DOM
 PS00134; TRYPSIN_HIS
 PS00135; TRYPSIN_SER 

PRINTS

 PR00722; CHYMOTRYPSIN.