CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006075
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate 5-kinase 
Protein Synonyms/Alias
 GK; Gamma-glutamyl kinase 
Gene Name
 PRO1 
Gene Synonyms/Alias
 YDR300C; D9740.12 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
297LNVPLHTKFIANDNKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline. 
Sequence Annotation
 DOMAIN 321 412 PUA.
 NP_BIND 175 176 ATP (By similarity).
 NP_BIND 219 225 ATP (By similarity).
 BINDING 56 56 Substrate (By similarity).
 BINDING 143 143 Substrate (By similarity).
 BINDING 155 155 Substrate; via amide nitrogen (By  
Keyword
 Amino-acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Proline biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 428 AA 
Protein Sequence
MKDANESKSY TIVIKLGSSS LVDEKTKEPK LAIMSLIVET VVKLRRMGHK VIIVSSGGIA 60
VGLRTMRMNK RPKHLAEVQA IAAIGQGRLI GRWDLLFSQF DQRIAQILLT RNDILDWTQY 120
KNAQNTINEL LNMGVIPIVN ENDTLSVREI KFGDNDTLSA ITSALIHADY LFLLTDVDCL 180
YTDNPRTNPD AMPILVVPDL SKGLPGVNTA GGSGSDVGTG GMETKLVAAD LATNAGVHTL 240
IMKSDTPANI GRIVEYMQTL ELDDENKVKQ AYNGDLTDLQ KREFEKLKAL NVPLHTKFIA 300
NDNKHHLKNR EFWILHGLVS KGAVVIDQGA YAALTRKNKA GLLPAGVIDV QGTFHELECV 360
DIKVGKKLPD GTLDPDFPLQ TVGKARCNYT SSELTKIKGL HSDQIEEELG YNDSEYVAHR 420
ENLAFPPR 428 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004349; F:glutamate 5-kinase activity; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006561; P:proline biosynthetic process; IDA:SGD. 
Interpro
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR001057; Glu/AcGlu_kinase.
 IPR011529; Glu_5kinase.
 IPR005715; Glu_5kinase/COase_Synthase.
 IPR019797; Glutamate_5-kinase_CS.
 IPR002478; PUA.
 IPR015947; PUA-like_domain. 
Pfam
 PF00696; AA_kinase
 PF01472; PUA 
SMART
 SM00359; PUA 
PROSITE
 PS00902; GLUTAMATE_5_KINASE
 PS50890; PUA 
PRINTS
 PR00474; GLU5KINASE.