UniProt Accession

 T2EA_HUMAN; P29083; Q16103 

Genbank Protein ID

 S67859; X63468 

Genbank Nucleotide ID

 AAB20413.1; CAA45068.1 

Protein Name

 General transcription factor IIE subunit 1 

Protein Synonyms/Alias

 General transcription factor IIE 56 kDa subunit; Transcription initiation factor IIE subunit alpha; TFIIE-alpha 

Gene Name

 GTF2E1 

Gene Synonyms/Alias

 TF2E1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	TEVPAALKRLAKYVI	ubiquitination	[1]
67	RSVLNNLKGDKFIKC	acetylation	[2]
104	RTLVNVVKYKLDHMR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. 

Sequence Annotation

 DOMAIN 14 104 HTH TFE/IIEalpha-type.
 ZN_FING 129 157 C4-type (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 67 67 N6-acetyllysine.
 MOD_RES 268 268 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Host-virus interaction; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 439 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR017919; TFIIE/TFIIEa_HTH.
 IPR002853; TFIIE_asu.
 IPR021600; TFIIE_asu_C.
 IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR013137; Znf_TFIIB. 

Pfam

 PF08271; TF_Zn_Ribbon
 PF11521; TFIIE-A_C-term
 PF02002; TFIIE_alpha 

SMART

 SM00531; TFIIE 

PROSITE

 PS51344; HTH_TFE_IIE 

PRINTS