UniProt Accession

 MTD1_YEAST; Q02046; D6VXE0 

Genbank Protein ID

 L02934; Z27116; Z28305; BK006944 

Genbank Nucleotide ID

 AAB00323.1; CAA81631.1; CAA82159.1; DAA09230.1 

Protein Name

 Methylenetetrahydrofolate dehydrogenase [NAD(+)] 

Protein Synonyms/Alias

  

Gene Name

 MTD1 

Gene Synonyms/Alias

 YKR080W; YKR400 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
118	QQVVCKEKDVEGLNH	acetylation	[1]
240	EYSEDLLKKCSLDSD	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis. 

Sequence Annotation

 NP_BIND 185 186 NAD.
 NP_BIND 208 209 NAD.
 NP_BIND 274 276 NAD.
 ACT_SITE 150 150 By similarity.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Nucleus; One-carbon metabolism; Oxidoreductase; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 320 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004487; F:methylenetetrahydrofolate dehydrogenase (NAD+) activity; IMP:SGD.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IMP:SGD.
 GO:0006730; P:one-carbon metabolic process; IDA:SGD.
 GO:0009113; P:purine nucleobase biosynthetic process; IMP:SGD.
 GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR016040; NAD(P)-bd_dom.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 

Pfam

 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 

SMART

  

PROSITE

  

PRINTS

 PR00085; THFDHDRGNASE.