UniProt Accession

 STK25_HUMAN; O00506; A8K7D2; Q15522; Q5BJF1 

Genbank Protein ID

 X99325; D63780; AK291947; BT019961; AC110299; DQ093965; CH471063; BC007852; BC091505 

Genbank Nucleotide ID

 CAA67700.1; BAA20420.1; BAF84636.1; AAV38764.1; AAY14683.1; AAY88740.1; EAW71265.1; AAH07852.1; AAH91505.1 

Protein Name

 Serine/threonine-protein kinase 25 

Protein Synonyms/Alias

 Ste20-like kinase; Sterile 20/oxidant stress-response kinase 1; SOK-1; Ste20/oxidant stress response kinase 1 

Gene Name

 STK25 

Gene Synonyms/Alias

 SOK1; YSK1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
22	DPEELFTKLDRIGKG	ubiquitination	[1]
142	RKIHRDIKAANVLLS	ubiquitination	[1, 2]
155	LSEQGDVKLADFGVA	ubiquitination	[1, 2]
171	QLTDTQIKRNTFVGT	ubiquitination	[1]
276	FITRYTKKTSFLTEL	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. 

Sequence Annotation

 DOMAIN 20 270 Protein kinase.
 NP_BIND 26 34 ATP (By similarity).
 ACT_SITE 140 140 Proton acceptor (By similarity).
 BINDING 49 49 ATP (By similarity).
 MOD_RES 174 174 Phosphothreonine; by autocatalysis.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Golgi apparatus; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 426 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0007163; P:establishment or maintenance of cell polarity; IEA:Compara.
 GO:0051645; P:Golgi localization; IEA:Compara.
 GO:0050772; P:positive regulation of axonogenesis; IEA:Compara.
 GO:0006979; P:response to oxidative stress; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS