CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019605
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-tubulin complex component 5 
Protein Synonyms/Alias
 GCP-5 
Gene Name
 TUBGCP5 
Gene Synonyms/Alias
 GCP5; KIAA1899 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
100NAPLPSIKEIKTDAHubiquitination[1]
410APRLSQLKVLHKVFSubiquitination[1]
526EKTENEEKMSDNASAubiquitination[2]
553HTMVSFLKPVLKQIIubiquitination[1]
564KQIIMAGKSMQLLKNubiquitination[1, 3]
570GKSMQLLKNLQCAESubiquitination[1, 2, 4]
590GARDAERKSLYTLFLubiquitination[1, 2, 5, 6]
622LTEQQATKENLMKMQubiquitination[1, 2]
663EQSDFHEKFAGGDVCubiquitination[1]
697CLYPHIDKQYLDCCGubiquitination[1]
788FENVDTAKKKLPVHIubiquitination[2]
789ENVDTAKKKLPVHILubiquitination[2]
856TAEKPRLKEGLIHEQubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1024 AA 
Protein Sequence
MARHGPPWSR LDAQQERDVR ELVRGVAGLQ DEADPNFQLA LNFAWSNFRF HRFLDVNSHK 60
IEKTIEGIYE KFVIHSDLSK AASWKRLTEE FLNAPLPSIK EIKTDAHYSI LSLLLCLSDS 120
PSNSSYVETP RNKEVEKKDD FDWGKYLMED EEMDIGPYMD TPNWSEESEE ENDQQPLSRE 180
DSGIQVDRTP LEEQDQNRKL DPCISWKDEP DDRSWLEHHV VHQYWTARPS QFPHSLHLHS 240
NLAAVWDQHL YSSDPLYVPD DRVLVTETQV IRETLWLLSG VKKLFIFQLI DGKVTVRNNI 300
IVTHLTHSCL RSVLEQIAAY GQVVFRLQEF IDEVMGHSSE SMLPGSGSVP KKSTEAPFRT 360
YQAFMWALYK YFISFKEELA EIEKCIINND TTITLAIVVD KLAPRLSQLK VLHKVFSTGV 420
AEVPPDTRNV VRASHLLNTL YKAILEYDNV GEASEQTVSL LFSLWVETVR PYLQTVDEWI 480
VHGHLWDGAR EFIIQRNKNV PVNHRDFWYA TYTLYSVSEK TENEEKMSDN ASASSGSDQG 540
PSSRQHTMVS FLKPVLKQII MAGKSMQLLK NLQCAESTTC QAGARDAERK SLYTLFLESV 600
QSRLRHGEDS TPQVLTEQQA TKENLMKMQS IAESHLELDD VHDPLLAINF ARMYLEQSDF 660
HEKFAGGDVC VDRSSESVTC QTFELTLRSC LYPHIDKQYL DCCGNLMQTL KKDYRLVEYL 720
QAMRNFFLME GGDTMYDFYT SIFDKIREKE TWQNVSFLNV QLQEAVGQRY PEDSSRLSIS 780
FENVDTAKKK LPVHILDGLT LSYKVPWPVD IVISLECQKI YNQVFLLLLQ IKWAKYSLDV 840
LLFGELVSTA EKPRLKEGLI HEQDTVAQFG PQKEPVRQQI HRMFLLRVKL MHFVNSLHNY 900
IMTRILHSTG LEFQHQVEEA KDLDQLIKIH YRYLSTIHDR CLLREKVSFV KEAIMKVLNL 960
ALMFADGWQA GLGTWRMESI EKMESDFKNC HMFLVTILNK AVCRGSFPHL ESLALSLMAG 1020
MEQS 1024 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008274; C:gamma-tubulin ring complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0000922; C:spindle pole; IEA:InterPro.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007020; P:microtubule nucleation; IDA:UniProtKB. 
Interpro
 IPR007259; Spc97_Spc98. 
Pfam
 PF04130; Spc97_Spc98 
SMART
  
PROSITE
  
PRINTS