CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016692
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc fingers and homeoboxes protein 3 
Protein Synonyms/Alias
 Triple homeobox protein 1; Zinc finger and homeodomain protein 3 
Gene Name
 Zhx3 
Gene Synonyms/Alias
 Kiaa0395; Tix1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
721ERKVSPIKINLKNLRubiquitination[1]
725SPIKINLKNLRVTEAubiquitination[1]
761LVEQPPSKVSYKKTAubiquitination[1]
815GDSRYALKNGQLKWYubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a transcriptional repressor. Involved in the early stages of mesenchymal stem cell (MSC) osteogenic differentiation. Is a regulator of podocyte gene expression during primary glomerula disease. Binds to promoter DNA. 
Sequence Annotation
 ZN_FING 77 100 C2H2-type 1.
 ZN_FING 109 132 C2H2-type 2.
 DNA_BIND 300 359 Homeobox 1.
 DNA_BIND 489 548 Homeobox 2.
 DNA_BIND 607 666 Homeobox 3.
 DNA_BIND 759 818 Homeobox 4.
 DNA_BIND 830 889 Homeobox 5.
 REGION 1 107 Required for nuclear localization (By
 REGION 238 483 Required for homodimerization and
 REGION 299 497 Required for repressor activity (By
 REGION 492 550 Required for nuclear localization (By
 MOD_RES 941 941 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Differentiation; DNA-binding; Homeobox; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 951 AA 
Protein Sequence
MASKRKSTTP CMIPVKTVVL PGASTEPQPV ESLPEGPQQD LPSEAPDASS EAAPNPSSTD 60
GSALANGHRS TLDGYVYCCK ECEFRSQDVT HFIGHMNSEH TDFNKDPTFV CTGCSFLAKN 120
PEGLSLHNAK CHSGEASFLW NVTKPDNHVV VEQSVPDSAS SSVLAGESTT EGTEIIITKT 180
PIMKIMKGKA EAKKIHMLKE NAPNQPGSEA LPKPLAGERE VKEGDHTFIN GAAPGSQASA 240
KSTKPPPAAN GPLIGTVPVL PAGIAQFLSL QQQPPVHAQH HTHQPLPTSK TLPKVMIPLS 300
SIPTYNAAMD SNSFLKNSFH KFPYPTKAEL CYLTVVTKYP EEQLKIWFTA QRLKQGISWS 360
PEEIEDARKK MFNTVIQSVP QPTITVLNTP LVASAGNVQH LIQATLPGHA VGQPEGTAGG 420
LLVTQPLMAN GLQASSSSLP LTTASVPKPT VAPINTVCSN SASAVKVVNA AQSLLTACPS 480
ITSQAFLDAN IYKNKKSHEQ LSALKGSFCR NQFPGQSEVE HLTKVTGLST REVRKWFSDR 540
RYHCRNLKGS RAMMPGEHGS VLIDSVPEVP FPLASKVPEV TCIPTATSLV SHPATKRQSW 600
HQTPDFTPTK YKERAPEQLR VLENSFAQNP LPPEEELDRL RSETKMTRRE IDGWFSERRK 660
KVNTEETKKA DGHMPKEEEE GAEQEGRDEE LANELRVPGE NGSPEMFLSH ALAERKVSPI 720
KINLKNLRVT EASGKSEFPG MGVCEPEEDG LNKLVEQPPS KVSYKKTAQQ RHLLRQLFVQ 780
TQWPSNQDYD SIMAQTGLPR PEVVRWFGDS RYALKNGQLK WYEDYKRGNF PPGLLVIAPG 840
NRELLQDYYM THKMLCEEDL QTLCDKTQMS AQQVKQWFAE KMGEETRAVA DISSEDQGPR 900
NGEPVAVHKV LGDAYSELSE NSESWEPSAP EASSEPFDTS SPQSGRQLEA D 951 
Gene Ontology
 GO:0005938; C:cell cortex; ISO:MGI.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0045669; P:positive regulation of osteoblast differentiation; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR015880; Znf_C2H2-like. 
Pfam
 PF00046; Homeobox 
SMART
 SM00389; HOX
 SM00355; ZnF_C2H2 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS