CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007089
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box protein MET30 
Protein Synonyms/Alias
 E3 ubiquitin ligase complex SCF(Met30) subunit MET30; Methionine-requiring protein 30 
Gene Name
 MET30 
Gene Synonyms/Alias
 YIL046W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
235CEQHIDRKCPNCGWGubiquitination[1]
249GLPLLHMKRARIQQNubiquitination[1]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Negatively regulates sulfur amino acids biosynthesis genes expression. Controls cell cycle function (being required for the G1/S transition and M-phase but not the S-phase), sulfur metabolism, and methionine biosynthesis as part of the E3 ubiquitin ligase complex SCF(Met30). Required for the efficient binding of CDC45 and MCM proteins to origins of replication. Involved in the S-adenosylmethionine (AdoMet)-mediated inhibition of the transcription function of MET4. In the context of the E3 ubiquitin ligase complex SCF(Met30), involved in the degradation of MET4 and the cellular response to cadmium. Required for efficient expression of G1 cyclins. 
Sequence Annotation
 DOMAIN 181 227 F-box.
 REPEAT 300 328 WD 1.
 REPEAT 340 368 WD 2.
 REPEAT 380 408 WD 3.
 REPEAT 419 449 WD 4.
 REPEAT 461 499 WD 5.
 REPEAT 509 538 WD 6.
 REPEAT 550 578 WD 7.
 REPEAT 607 635 WD 8.
 REGION 1 299 Necessary to mediate nuclear
 REGION 180 277 Important for mediating
 REGION 180 225 Interaction with SKP1/CBF3D.
 REGION 277 640 Interaction with MET4.
 MOD_RES 67 67 Phosphoserine.  
Keyword
 Amino-acid biosynthesis; Cell cycle; Complete proteome; Cysteine biosynthesis; Cytoplasm; Methionine biosynthesis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 640 AA 
Protein Sequence
MRRERQRMMS FEDKDKDDLD NSNSNNSSEM TDTAMMPPLK RLLITGSSDD LAQGSSGKKK 60
MTMATRSPSS SPDLATNDSG TRVQPLPEYN FTKFCYRHNP DIQFSPTHTA CYKQDLKRTQ 120
EINANIAKLP LQEQSDIHHI ISKYSNSNDK IRKLILDGIL STSCFPQLSY ISSLVTHMIK 180
IDFISILPQE LSLKILSYLD CQSLCNATRV CRKWQKLADD DRVWYHMCEQ HIDRKCPNCG 240
WGLPLLHMKR ARIQQNSTGS SSNADIQTQT TRPWKVIYRE RFKVESNWRK GHCRIQEFKG 300
HMDGVLTLQF NYRLLFTGSY DSTIGIWDLF TGKLIRRLSG HSDGVKTLYF DDRKLITGSL 360
DKTIRVWNYI TGECISTYRG HSDSVLSVDS YQKVIVSGSA DKTVKVWHVE SRTCYTLRGH 420
TEWVNCVKLH PKSFSCFSCS DDTTIRMWDI RTNSCLKVFR GHVGQVQKII PLTIKDVENL 480
ATDNTSDGSS PQDDPTMTDG ADESDTPSNE QETVLDENIP YPTHLLSCGL DNTIKLWDVK 540
TGKCIRTQFG HVEGVWDIAA DNFRIISGSH DGSIKVWDLQ SGKCMHTFNG RRLQRETQHT 600
QTQSLGDKVA PIACVCIGDS ECFSGDEFGC VKMYKFDLND 640 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043224; C:nuclear SCF ubiquitin ligase complex; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0000209; P:protein polyubiquitination; IMP:SGD.
 GO:0030174; P:regulation of DNA-dependent DNA replication initiation; IMP:SGD.
 GO:0000114; P:regulation of transcription involved in G1 phase of mitotic cell cycle; IMP:SGD.
 GO:0046685; P:response to arsenic-containing substance; IDA:SGD.
 GO:0046686; P:response to cadmium ion; IDA:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IGI:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00646; F-box
 PF00400; WD40 
SMART
 SM00256; FBOX
 SM00320; WD40 
PROSITE
 PS50181; FBOX
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.