CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029081
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Zinc finger protein 281 
Protein Synonyms/Alias
  
Gene Name
 ZNF281 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66APDMTFKKEPAASAAubiquitination[1, 2]
177HHGTEEPKQDTNVKKubiquitination[2]
279EKIHSREKPFGCDQCubiquitination[1, 2]
293CSMKFIQKYHMERHKubiquitination[1, 2]
307KRTHSGEKPYKCDTCubiquitination[1]
310HSGEKPYKCDTCQQYubiquitination[1, 2]
337TCGEVIVKGATSAEPubiquitination[1, 2]
373SRRKTKSKSIAIENKubiquitination[2]
380KSIAIENKEQKTGKTubiquitination[2]
383AIENKEQKTGKTNESubiquitination[1]
437IFKKGSRKNTDKNYLubiquitination[2]
441GSRKNTDKNYLNFVSubiquitination[1, 2]
462GQKSLSGKPSGSLGIubiquitination[1, 2]
503DDAMQFSKKRRYLPTubiquitination[1, 2, 3, 4]
504DAMQFSKKRRYLPTAubiquitination[2]
563EIKSCHDKSGIPDEVubiquitination[1, 2]
581ILDQYSNKSESQKEDubiquitination[1, 2, 4, 5]
586SNKSESQKEDPFNIAubiquitination[1, 2, 3, 4, 5]
625TQTPSNDKASMLQEYubiquitination[1, 2, 3, 5]
634SMLQEYSKYLQQAFEubiquitination[1, 2, 3, 4]
751AFQSSSQKLTSQKEQacetylation[6]
751AFQSSSQKLTSQKEQubiquitination[1, 2, 3, 4]
759LTSQKEQKNLESSTGubiquitination[2, 3]
782ASQIDPQKDIEPRTTubiquitination[1, 2, 4]
804QAFGSQFKSGSRVPMubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 859 AA 
Protein Sequence
MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQAP AAEPPPPPAP 60
DMTFKKEPAA SAAAFPSQRT SWGFLQSLVS IKQEKPADPE EQQSHHHHHH HHYGGLFAGA 120
EERSPGLGGG EGGSHGVIQD LSILHQHVQQ QPAQHHRDVL LSSSSRTDDH HGTEEPKQDT 180
NVKKAKRPKP ESQGIKAKRK PSASSKPSLV GDGEGAILSP SQKPHICDHC SAAFRSSYHL 240
RRHVLIHTGE RPFQCSQCSM GFIQKYLLQR HEKIHSREKP FGCDQCSMKF IQKYHMERHK 300
RTHSGEKPYK CDTCQQYFSR TDRLLKHRRT CGEVIVKGAT SAEPGSSNHT NMGNLAVLSQ 360
GNTSSSRRKT KSKSIAIENK EQKTGKTNES QISNNINMQS YSVEMPTVSS SGGIIGTGID 420
ELQKRVPKLI FKKGSRKNTD KNYLNFVSPL PDIVGQKSLS GKPSGSLGIV SNNSVETIGL 480
LQSTSGKQGQ ISSNYDDAMQ FSKKRRYLPT ASSNSAFSIN VGHMVSQQSV IQSAGVSVLD 540
NEAPLSLIDS SALNAEIKSC HDKSGIPDEV LQSILDQYSN KSESQKEDPF NIAEPRVDLH 600
TSGEHSELVQ EENLSPGTQT PSNDKASMLQ EYSKYLQQAF EKSTNASFTL GHGFQFVSLS 660
SPLHNHTLFP EKQIYTTSPL ECGFGQSVTS VLPSSLPKPP FGMLFGSQPG LYLSALDATH 720
QQLTPSQELD DLIDSQKNLE TSSAFQSSSQ KLTSQKEQKN LESSTGFQIP SQELASQIDP 780
QKDIEPRTTY QIENFAQAFG SQFKSGSRVP MTFITNSNGE VDHRVRTSVS DFSGYTNMMS 840
DVSEPCSTRV KTPTSQSYR 859 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS