CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004358
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional purine biosynthesis protein PurH 
Protein Synonyms/Alias
 Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase 
Gene Name
 purH 
Gene Synonyms/Alias
 b4006; JW3970 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
17ALLSVSDKAGIVEFAacetylation[1]
47TARLLAEKGLPVTEVacetylation[1]
158SDYDAIIKEMDDNEGacetylation[1]
222TLNLNFIKKLDMRYGacetylation[1]
245FYIEENVKEASVATAacetylation[1]
258TATQVQGKALSYNNIacetylation[1]
287EPACVIVKHANPCGVacetylation[1, 2]
309DAYDRAYKTDPTSAFacetylation[1]
359ALKITAAKQNVRVLTacetylation[1]
380RVPGLDFKRVNGGLLacetylation[1]
422RDALFCWKVAKFVKSacetylation[1]
455MSRVYSAKIAGIKAAacetylation[1]
469ADEGLEVKGSSMASDacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 
Functional Description
  
Sequence Annotation
 MOD_RES 287 287 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Multifunctional enzyme; Purine biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 529 AA 
Protein Sequence
MQQRRPVRRA LLSVSDKAGI VEFAQALSAR GVELLSTGGT ARLLAEKGLP VTEVSDYTGF 60
PEMMDGRVKT LHPKVHGGIL GRRGQDDAIM EEHQIQPIDM VVVNLYPFAQ TVAREGCSLE 120
DAVENIDIGG PTMVRSAAKN HKDVAIVVKS SDYDAIIKEM DDNEGSLTLA TRFDLAIKAF 180
EHTAAYDSMI ANYFGSMVPA YHGESKEAAG RFPRTLNLNF IKKLDMRYGE NSHQQAAFYI 240
EENVKEASVA TATQVQGKAL SYNNIADTDA ALECVKEFAE PACVIVKHAN PCGVAIGNSI 300
LDAYDRAYKT DPTSAFGGII AFNRELDAET AQAIISRQFV EVIIAPSASE EALKITAAKQ 360
NVRVLTCGQW GERVPGLDFK RVNGGLLVQD RDLGMVGAEE LRVVTKRQPS EQELRDALFC 420
WKVAKFVKSN AIVYAKNNMT IGIGAGQMSR VYSAKIAGIK AADEGLEVKG SSMASDAFFP 480
FRDGIDAAAA AGVTCVIQPG GSIRDDEVIA AADEHGIAML FTDMRHFRH 529 
Gene Ontology
 GO:0003937; F:IMP cyclohydrolase activity; IGI:EcoCyc.
 GO:0004643; F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity; IGI:EcoCyc.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR024051; AICAR_Tfase_dom.
 IPR002695; AICARFT_IMPCHas.
 IPR016193; Cytidine_deaminase-like.
 IPR011607; MGS-like_dom. 
Pfam
 PF01808; AICARFT_IMPCHas
 PF02142; MGS 
SMART
 SM00798; AICARFT_IMPCHas
 SM00851; MGS 
PROSITE
  
PRINTS