CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007855
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Yorkie homolog 
Protein Synonyms/Alias
 65 kDa Yes-associated protein; YAP65 
Gene Name
 YAP1 
Gene Synonyms/Alias
 YAP65 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
497LAATKLDKESFLTWLubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). 
Sequence Annotation
 DOMAIN 171 204 WW 1.
 DOMAIN 230 263 WW 2.
 REGION 291 504 Transactivation domain.
 MOD_RES 61 61 Phosphoserine; by LATS1 and LATS2.
 MOD_RES 63 63 Phosphothreonine.
 MOD_RES 109 109 Phosphoserine; by LATS1 and LATS2.
 MOD_RES 110 110 Phosphothreonine (By similarity).
 MOD_RES 119 119 Phosphothreonine; by MAPK8 and MAPK9.
 MOD_RES 127 127 Phosphoserine; by LATS1 and LATS2.
 MOD_RES 128 128 Phosphoserine (By similarity).
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 138 138 Phosphoserine; by MAPK8 and MAPK9.
 MOD_RES 154 154 Phosphothreonine; by MAPK8 and MAPK9.
 MOD_RES 164 164 Phosphoserine; by LATS1 and LATS2.
 MOD_RES 274 274 Phosphoserine.
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 367 367 Phosphoserine; by MAPK8 and MAPK9.
 MOD_RES 371 371 Phosphoserine.
 MOD_RES 397 397 Phosphoserine; by LATS1 and LATS2.
 MOD_RES 400 400 Phosphoserine; by CK1.
 MOD_RES 403 403 Phosphoserine; by CK1.
 MOD_RES 407 407 Phosphotyrosine; by ABL1.
 MOD_RES 412 412 Phosphothreonine; by MAPK8 and MAPK9.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 504 AA 
Protein Sequence
MDPGQQPPPQ PAPQGQGQPP SQPPQGQGPP SGPGQPAPAA TQAAPQAPPA GHQIVHVRGD 60
SETDLEALFN AVMNPKTANV PQTVPMRLRK LPDSFFKPPE PKSHSRQAST DAGTAGALTP 120
QHVRAHSSPA SLQLGAVSPG TLTPTGVVSG PAATPTAQHL RQSSFEIPDD VPLPAGWEMA 180
KTSSGQRYFL NHIDQTTTWQ DPRKAMLSQM NVTAPTSPPV QQNMMNSASG PLPDGWEQAM 240
TQDGEIYYIN HKNKTTSWLD PRLDPRFAMN QRISQSAPVK QPPPLAPQSP QGGVMGGSNS 300
NQQQQMRLQQ LQMEKERLRL KQQELLRQAM RNINPSTANS PKCQELALRS QLPTLEQDGG 360
TQNPVSSPGM SQELRTMTTN SSDPFLNSGT YHSRDESTDS GLSMSSYSVP RTPDDFLNSV 420
DEMDTGDTIN QSTLPSQQNR FPDYLEAIPG TNVDLGTLEG DGMNIEGEEL MPSLQEALSS 480
DILNDMESVL AATKLDKESF LTWL 504 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IDA:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0008283; P:cell proliferation; IDA:UniProtKB.
 GO:0071480; P:cellular response to gamma radiation; IDA:UniProtKB.
 GO:0060242; P:contact inhibition; IDA:UniProtKB.
 GO:0003143; P:embryonic heart tube morphogenesis; IEA:Compara.
 GO:0035329; P:hippo signaling cascade; TAS:UniProtKB.
 GO:0030216; P:keratinocyte differentiation; IEA:Compara.
 GO:0048368; P:lateral mesoderm development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0030857; P:negative regulation of epithelial cell differentiation; IEA:Compara.
 GO:0030903; P:notochord development; IEA:Compara.
 GO:0048339; P:paraxial mesoderm development; IEA:Compara.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0046622; P:positive regulation of organ growth; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0010837; P:regulation of keratinocyte proliferation; IEA:Compara.
 GO:0072091; P:regulation of stem cell proliferation; IEA:Compara.
 GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB.
 GO:0035019; P:somatic stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0001570; P:vasculogenesis; IEA:Compara. 
Interpro
 IPR001202; WW_dom. 
Pfam
 PF00397; WW 
SMART
 SM00456; WW 
PROSITE
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS