CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022901
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Testin 
Protein Synonyms/Alias
 TESS 
Gene Name
 TES 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71GKLFEDTKYTTLIAKubiquitination[1]
88SDGIPMYKRNVMILTubiquitination[2]
102TNPVAAKKNVSINTVubiquitination[1]
134MQMLPKEKQPVAGSEubiquitination[2]
244YCCKLSMKEGDPAIYubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. 
Sequence Annotation
 DOMAIN 92 199 PET.
 DOMAIN 234 297 LIM zinc-binding 1.
 DOMAIN 299 359 LIM zinc-binding 2.
 DOMAIN 362 421 LIM zinc-binding 3.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Complete proteome; Cytoplasm; LIM domain; Metal-binding; Polymorphism; Reference proteome; Repeat; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE HDVLLSNEED 60
RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA KKNVSINTVT YEWAPPVQNQ 120
ALARQYMQML PKEKQPVAGS EGAQYRKKQL AKQLPAHDQD PSKCHELSPR EVKEMEQFVK 180
KYKSEALGVG DVKLPCEMDA QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK 240
LSMKEGDPAI YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR 300
CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK PCYVKNHAVV 360
CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG QKFMPVEGMV FCSVECKKRM 420
S 421 
Gene Ontology
 GO:0030054; C:cell junction; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB. 
Interpro
 IPR010442; PET_domain.
 IPR001781; Znf_LIM. 
Pfam
 PF00412; LIM
 PF06297; PET 
SMART
 SM00132; LIM 
PROSITE
 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2
 PS51303; PET 
PRINTS